Anti-Hepatocyte(2H5) Monoclonal, ALEXA Fluor 594
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Catalog number
GENTObsm-2741M-A594
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Price
Please ask
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Size
100 microliters
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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR 594
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Host organism
Mouse (Mus musculus)
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Target Protein Peptide
Hepatocyte
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Specificity
Highly specific antibody against Hepatocyte
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Modification
Not modified
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Clonality
Monoclonal Antibodies
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Clone
2H5
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Concentration
1ug per 1ul
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Antigen Source
human liver extract
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Gene ID
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross Reactive species
Human (Homo sapiens)
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Cross Reactive Species details
No significant cross reactivity is observed
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Background information
Hepatocyte specific antigen is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. Hepatcoyte labels an antigen in the mitochondrial fraction of the liver homogenates. Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes. HSA (Hepatocyte Specific Antigen) has been demonstrated consistently in the vast majority of hepatocellular carcinomas. Studies have shown the utility of HSA in the differential diagnosis of hepatocellular carcinoma, cholangiocarcinoma and hepatoblastomas.
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Purification method
Purified by Protein G.
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Storage
Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
Hepatocyte specic antigen; Cell growth inhibiting protein 42; Growth inhibiting protein 20; Human ALB; Human serum albumin.
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Alternative name 1
Hepatocyte(2H5) Monoclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative name 2
Hepatocyte(2H5) Monoclonal anti-, ALEXA FLUOR 594 Conjugated
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Advisory
Cycles of freezing and thawing should be avoided as such cycles may denature the peptide chains of the antibody and reduce its affinity, specificity and reactivity. For antibodies in a liquid form or reconstituted lyophilized antibodies - small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Gene target
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Short name
Anti-Hepatocyte(2H5) Monoclonal,
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Technique
anti-, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 594
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Alternative name
Antibody toHepatocyte(2H5) monoclonal, ALEXA Fluor 594
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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