Recombinant Human Hyaluronidase PH-20 (SPAM1)

Estimated Turnaround Time

6-11 business days
Gene Name

SPAM1
Alternative Names

Hyaluronoglucosaminidase PH-20Sperm adhesion molecule 1Sperm surface protein PH-20
Source

E.coli
Purity

> 90% by SDS-PAGE
Shipping Conditions

Ice packs
Storage conditions

Short term: -20°C; Long term: -80°C. Minimize freeze and thaw cycles.
Shelf life

12 months in lyophilized form; 6 months in solution form.
Restriction

For research use only. Not for diagnostic procedures.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source

Recombinants or rec. proteins
Group

recombinants

Gene target

Hyaluronidase PH-20 SPAM1
Gene symbol

SPAM1, PAH, PIRC103, SNORD114-20, SNORD116-20, IGLVI-20, PIRC104, IGHV3-20
Short name

Recombinant Hyaluronidase PH-20 (SPAM1)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biomatik advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Species

Homo sapiens (Human), Humans
Alternative name

Rec. H. sapiens Hyaluronidase PH-20 (sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding))
Alternative technique

rec
Alternative to gene target

sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), HEL-S-96n and HYA1 and HYAL1 and HYAL3 and HYAL5 and PH-20 and PH20 and SPAG15, SPAM1 and IDBG-39033 and ENSG00000106304 and 6677, hyalurononglucosaminidase activity, Plasma membranes

Identity

HGNC:11217
Gene

SPAM1
Long gene name

sperm adhesion molecule 1
Synonyms gene name
- sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
Synonyms
- HYAL5
- PH-20
- SPAG15
Synonyms name
- PH-20 hyaluronidase
GenBank acession
- L13781
Locus

7q31
Discovery year

1995-07-18
Pubmed identfication
- 8282124
- 8575780
Classification
- Hyaluronidases
VEGA ID

OTTHUMG00000157284

Identity

HGNC:5934
Gene

IGLVI-20
Long gene name

immunoglobulin lambda variable (I)-20 (pseudogene)
Synonyms gene name
- immunoglobulin lambda variable (I)-20
- immunoglobulin lambda variable (I)-20 pseudogene
Synonyms
- IGLV(I)-20
GenBank acession
- D87007
Locus

22q11.22
Discovery year

2000-05-08
Entrez gene record

28769
RefSeq identity
- NG_000002
Classification
- Immunoglobulin lambda locus at 22q11.2
VEGA ID

OTTHUMG00000151242

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data