Rat Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) CLIA Kit, 96 well plate
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Catalog numberEKU09860
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Price:
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Size5x96T
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Verified reactivityRattus norvegicus (Rat)
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Detection limits234.38-15000 pg/mL
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Sensitivity limit7,3 pg/mL
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Samples to be analyzedSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
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Assay duration2 hours, 40min
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Verified applicationsCLIA
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Assay classSandwich
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ELISA detectionChemiluminescent
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Protein numberQ00981
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Alternate protein numberPlease refer to SwissProt
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Gene number29545
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Gene nameUbiquitin Carboxyl Terminal Hydrolase L1
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Alternate gene namePARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). No significant cross-reactivity or interference between Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) level in the sample or standard.;
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaEnzyme & Kinase;
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WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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DescriptionA microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Gene symbolUCHL1-DT, UCHL1
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Short nameUbiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) CLIA Kit, 96
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HostRat
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SpeciesRat, Rats
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Alternative nameRat Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) CLIA reagent, 96 well plate
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Alternative techniquekits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameUCHL1 divergent transcript
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Synonyms gene
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Synonyms gene name
- UCHL1 antisense RNA 1 (head to head)
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GenBank acession
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Locus
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Discovery year2012-12-01
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Entrez gene record
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RefSeq identity
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Classification
- Divergent transcripts
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L1
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Synonyms gene
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Synonyms gene name
- ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
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VEGA ID