Rat Transferrin (TRF) CLIA Kit, 96 well plate
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Catalog numberEKU08953
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Price:
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Size5x96T
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Verified reactivityRattus norvegicus (Rat)
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Detection limits156.25-40000 pg/mL
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Sensitivity limit20,3 pg/mL
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Samples to be analyzedSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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Assay duration2 hours, 40min
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Verified applicationsCLIA
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Assay classSandwich
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ELISA detectionChemiluminescent
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Protein numberP12346
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Alternate protein numberPlease refer to SwissProt
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Gene numberPlease refer to GenBank
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Gene nameTransferrin
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Alternate gene nameTF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Transferrin (TRF). No significant cross-reactivity or interference between Transferrin (TRF) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Transferrin (TRF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transferrin (TRF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transferrin (TRF) level in the sample or standard.;
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin (TRF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin (TRF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaTumor immunity;
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WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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DescriptionA microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Gene symbolTRF-GAA1-1, IL5, TRF-GAA1-4, TRF-GAA1-2, TRF-GAA1-6, TRF-GAA1-3, TRF-GAA1-5, TERF1, TRF-GAA12-1, TRF-GAA7-1
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Short nameTransferrin (TRF) CLIA Kit, 96
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HostRat
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SpeciesRat, Rats
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Alternative nameRat Transferrin (TRF) CLIA reagent, 96 well plate
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Alternative techniquekits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nametRNA-Phe (anticodon GAA) 1-1
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Synonyms gene
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Synonyms gene name
- transfer RNA phenylalanine 3 (anticodon GAA)
- transfer RNA-Phe (GAA) 1-5
- transfer RNA-Phe (GAA) 1-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nameinterleukin 5
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Synonyms gene name
- interleukin 5 (colony-stimulating factor, eosinophil)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Phe (anticodon GAA) 1-4
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Synonyms gene
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Synonyms gene name
- transfer RNA phenylalanine 2 (anticodon GAA)
- transfer RNA-Phe (GAA) 1-2
- transfer RNA-Phe (GAA) 1-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-01
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Entrez gene record
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Pubmed identfication
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Phe (anticodon GAA) 1-2
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Synonyms gene
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Synonyms gene name
- transfer RNA phenylalanine 7 (anticodon GAA)
- transfer RNA-Phe (GAA) 1-6
- transfer RNA-Phe (GAA) 1-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Phe (anticodon GAA) 1-6
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Synonyms gene
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Synonyms gene name
- transfer RNA phenylalanine 10 (anticodon GAA)
- transfer RNA-Phe (GAA) 1-4
- transfer RNA-Phe (GAA) 1-6
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Phe (anticodon GAA) 1-3
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Synonyms gene
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Synonyms gene name
- transfer RNA phenylalanine 11 (anticodon GAA)
- transfer RNA-Phe (GAA) 1-1
- transfer RNA-Phe (GAA) 1-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Phe (anticodon GAA) 1-5
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Synonyms gene
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Synonyms gene name
- transfer RNA phenylalanine 6 (anticodon GAA)
- transfer RNA-Phe (GAA) 1-3
- transfer RNA-Phe (GAA) 1-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametelomeric repeat binding factor 1
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Synonyms gene
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Synonyms gene name
- telomeric repeat binding factor (NIMA-interacting) 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Shelterin complex
- Myb/SANT domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Phe (anticodon GAA) 12-1
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Synonyms gene
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Synonyms gene name
- transfer RNA phenylalanine 14 (anticodon GAA) pseudogene
- transfer RNA-Phe (GAA) 12-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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RefSeq identity
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Classification
- Cytoplasmic transfer RNA pseudogenes
Gene info
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Identity
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Gene
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Long gene nametRNA-Phe (anticodon GAA) 7-1
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Synonyms gene
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Synonyms gene name
- transfer RNA phenylalanine 12 (anticodon GAA)
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNA pseudogenes