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Verified reactivity
Rattus norvegicus (Rat)
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Detection limits
7.81-2000 pg/mL
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Sensitivity limit
2,65 pg/mL
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Samples to be analyzed
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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Assay duration
2 hours
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Verified applications
CLIA
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Assay class
Competitive
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ELISA detection
Chemiluminescent
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Protein number
P04089
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Alternate protein number
Please refer to SwissProt
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Gene number
24694
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Gene name
Parathyroid Hormone
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Alternate gene name
iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin
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Estimated production time
7-11 business days
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Shipping requirements
Blue ice
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Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for
8 months
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ELISA s specificity
This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH). No significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.
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ELISA s cross reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Parathyroid Hormone (PTH). A competitive inhibition reaction is launched between biotin labeled Parathyroid Hormone (PTH) and unlabeled Parathyroid Hormone (PTH) (Standards or samples) with the pre-coated antibody specific to Parathyroid Hormone (PTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Parathyroid Hormone (PTH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Parathyroid Hormone (PTH) level in the sample or standard.
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Protocol
Please see ELISA's datasheet, otherwise contact us
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Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main area
Metabolic pathway;Endocrinology;Hormone metabolism;
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Warnings
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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Notes
For research use only. Not for diagnostic procedures.
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Description
Hormone releasing factors and releasing hormones are signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells. A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
