-
Verified reactivity
Mus musculus (Mouse)
-
Detection limits
156.25-10000 pg/mL
-
Sensitivity limit
6,45 pg/mL
-
Samples to be analyzed
Tissue homogenates and other biological fluids
-
Assay duration
2 hours, 40min
-
Verified applications
CLIA
-
Assay class
Sandwich
-
ELISA detection
Chemiluminescent
-
Protein number
Q9R0G7
-
Alternate protein number
Please refer to SwissProt
-
Gene number
24136
-
Gene name
Zinc Finger Homeobox Protein 1B
-
Alternate gene name
ZEB2; SIP1; SIP-1; SMADIP1; SIP1; Zinc Finger E-Box Binding Homeobox 2; SMAD Interacting Protein 1
-
Estimated production time
7-11 business days
-
Shipping requirements
Blue ice
-
Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
-
Use for
8 months
-
ELISA s specificity
This assay has high sensitivity and excellent specificity for detection of Zinc Finger Homeobox Protein 1B (ZFHX1B). No significant cross-reactivity or interference between Zinc Finger Homeobox Protein 1B (ZFHX1B) and analogues was observed.
-
ELISA s cross reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
-
Assay principle
The microplate provided in this kit has been pre-coated with an antibody specific to Zinc Finger Homeobox Protein 1B (ZFHX1B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Zinc Finger Homeobox Protein 1B (ZFHX1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Zinc Finger Homeobox Protein 1B (ZFHX1B) level in the sample or standard.;
-
Protocol
Please see ELISA's datasheet, otherwise contact us
-
Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
-
ELISA s stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
-
QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
-
Research main area
Immune molecule;
-
Warnings
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
-
Notes
For research use only. Not for diagnostic procedures.
-
-
Description
Finger like and finger proteins are like zinc fingers small proteins with a structural motif that is characterized by the coordination of one or more zinc ions in order to stabilize the fold. Homeobox and homeodomain families. A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
