iScript One-Step qRT-PCR Kit without Dye, 100Rxns-20ul
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Catalog number
A4226
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Price
Please ask
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Size
1.25ML(100Rxns)
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Chemical grade
Molecular Biology
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Storage requirements
-20 °C
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Category
DNA & PCR
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Hazardous number
Please see datasheet or contact us for more information and details about hazardous materials.
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Chemical class
Please see datasheet
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Chemical PG
No Data Available
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Supplementary technical information
Contact us for more technical information
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Notes
For research use only. Not for diagnostic procedures.
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short name
iScript One-Step qRT-PCR Kit without Dye, 100Rxns-20ul
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Technique
step, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. steps
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Label
dye
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Alternative name
iScript One-Step qRT-PCR test kit reagent without Dye, 100Rxns-20ul
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Alternative technique
steps, kits, dna-amplification
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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