Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit, 96 well plate
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Catalog numberEKU07360
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Price:
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Size96T
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Verified reactivityHomo sapiens (Human)
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Detection limits0.156-10 ng/mL
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Sensitivity limit0,063 ng/mL
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Samples to be analyzedTissue homogenates, cell lysates and other biological fluids.
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Assay duration3 hours
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Verified applicationsELISA
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Assay classSandwich
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ELISA detectionColorimetric
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Protein numberPlease refer to Uniprot
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Alternate protein numberPlease refer to SwissProt
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Gene numberPlease refer to GenBank
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Gene nameSix Transmembrane Epithelial Antigen Of The Prostate 2
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Alternate gene nameIPCA1; PCANAP1; PUMPCn; STAMP1; STMP; Prostate Cancer Associated Protein 1; Protein up-regulated in metastatic prostate cancer; SixTransMembrane protein of prostate 1
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2). No significant cross-reactivity or interference between Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaSignal transduction;
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WarningsThe Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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DescriptionAntigens are peptides or recombinant or native dependent on the production method. A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbolSTEAP2-AS1, STEAP2
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Short nameSix Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit, 96
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TechniqueELISA kit, antigen, ELISA, antigenes, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesHuman, Humans
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Alternative nameH. sapiens Six Transmembrane Epithelial protein on The Prostate 2 (STEAP family member 2, metalloreductase) Enzyme-linked immunosorbent assay reagent, 96 well plate
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Alternative techniqueantigenes, elisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissueprostate
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Gene info
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Identity
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Gene
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Long gene nameSTEAP2 antisense RNA 1
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Synonyms gene name
- STEAP2 antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2011-09-30
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Entrez gene record
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSTEAP2 metalloreductase
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Synonyms gene
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Synonyms gene name
- prostate cancer associated protein 1
- six transmembrane epithelial antigen of the prostate 2
- STEAP family member 2, metalloreductase
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-09-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- STEAP family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data