Human Serum Amyloid P Component (SAP) CLIA Kit, 96 well plate
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Catalog numberEKU09774
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PricePlease ask
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Size10x96T
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Verified reactivityHomo sapiens (Human)
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Detection limits1.56-400 ng/mL
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Sensitivity limit0,23 ng/mL
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Samples to be analyzedSerum, plasma and other biological fluids
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Assay duration2 hours, 40min
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Verified applicationsCLIA
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Assay classSandwich
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ELISA detectionChemiluminescent
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Protein numberPlease refer to Uniprot
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Alternate protein numberPlease refer to SwissProt
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Gene numberPlease refer to GenBank
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Gene nameSerum Amyloid P Component
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Alternate gene nameAPCS; PTX2; Pentaxin-Related; 9.5S Alpha-1-Glycoprotein
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Serum Amyloid P Component (SAP). No significant cross-reactivity or interference between Serum Amyloid P Component (SAP) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid P Component (SAP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Serum Amyloid P Component (SAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Serum Amyloid P Component (SAP) level in the sample or standard.;
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Amyloid P Component (SAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Amyloid P Component (SAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaInfection immunity;
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WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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DescriptionA microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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GroupSera
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AboutSerum should be stored at +5°C
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Gene target
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Gene symbolGM2A, PTPRH, APCS, DLG1, DLG4, SH2D1A, DLG3
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Short nameSerum Amyloid P Component (SAP) CLIA Kit, 96
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Techniqueserum
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SpeciesHuman, Humans
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Alternative nameH. sapiens blood serum Amyloid P Component (SAP) CLIA reagent, 96 well plate
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Alternative techniquesera, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissueserum
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Gene info
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Identity
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Gene
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Long gene nameGM2 ganglioside activator
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Synonyms gene name
- GM2 ganglioside activator protein
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Synonyms
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type H
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Synonyms
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Locus
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Discovery year1994-09-14
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Entrez gene record
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Pubmed identfication
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameamyloid P component, serum
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Short pentraxins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namediscs large MAGUK scaffold protein 1
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Synonyms gene name
- discs, large homolog 1 (Drosophila)
- discs large homolog 1, scribble cell polarity complex component
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-05-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Membrane associated guanylate kinases
- PDZ domain containing
- MicroRNA protein coding host genes
- Scribble complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namediscs large MAGUK scaffold protein 4
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Synonyms gene name
- discs, large homolog 4 (Drosophila)
- discs large homolog 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-11-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Membrane associated guanylate kinases
- PDZ domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSH2 domain containing 1A
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Synonyms gene
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Synonyms gene name
- lymphoproliferative syndrome
- SH2 domain protein 1A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SH2 domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namediscs large MAGUK scaffold protein 3
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Synonyms gene name
- discs, large homolog 3 (neuroendocrine-dlg, Drosophila)
- discs, large homolog 3 (Drosophila)
- discs large homolog 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Membrane associated guanylate kinases
- PDZ domain containing
- Protein phosphatase 1 regulatory subunits
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data