Human Pulmonary Activation Regulated Chemokine (PARC) CLIA Kit, 96 well plate
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Catalog numberEKU09665
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Price:
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Size5x96T
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Verified reactivityHomo sapiens (Human)
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Detection limits3.12-200 ng/mL
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Sensitivity limit0,13 ng/mL
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Samples to be analyzedSerum, plasma and other biological fluids
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Assay duration2 hours, 40min
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Verified applicationsCLIA
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Assay classSandwich
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ELISA detectionChemiluminescent
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Protein numberP55774
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Alternate protein numberPlease refer to SwissProt
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Gene number6362
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Gene namePulmonary Activation Regulated Chemokine
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Alternate gene nameCCL18; AMAC1; SCYA18; DCCK1; MIP-4; CKb7; Alternative Macrophage Activation Associated CC Chemokine 1; Chemokine C-C-Motif Ligand 18; Macrophage inflammatory protein 4
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Pulmonary Activation Regulated Chemokine (PARC). No significant cross-reactivity or interference between Pulmonary Activation Regulated Chemokine (PARC) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Pulmonary Activation Regulated Chemokine (PARC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pulmonary Activation Regulated Chemokine (PARC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pulmonary Activation Regulated Chemokine (PARC) level in the sample or standard.;
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pulmonary Activation Regulated Chemokine (PARC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pulmonary Activation Regulated Chemokine (PARC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaCytokine;
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WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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DescriptionChemokines, chemokine receptors, ligands , motif chemokines and cytokines are supplied by bioma in 1. A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbolCUL9, CCL18
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Short namePulmonary Activation Regulated Chemokine (PARC) CLIA Kit, 96
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Techniqueactivation
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SpeciesHuman, Humans
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Alternative nameH. sapiens Pulmonary Activation Regulated Chemokine (PARC) CLIA reagent, 96 well plate
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Alternative techniquekits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namecullin 9
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2009-01-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RBR E3 ubiquitin ligases
- Cullins
- Armadillo like helical domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 18
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated
- chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
- chemokine (C-C motif) ligand 18
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-01-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID