Human Myelin Basic Protein (MBP) CLIA Kit, 96 well plate
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Catalog numberEKU08877
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Price:
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Size96T
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Verified reactivityHomo sapiens (Human)
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Detection limits117.19-30000 pg/mL
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Sensitivity limit17,5 pg/mL
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Samples to be analyzedSerum, plasma, cerebrospinal fluid and other biological fluids
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Assay duration2 hours, 40min
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Verified applicationsCLIA
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Assay classSandwich
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ELISA detectionChemiluminescent
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Protein numberP02686
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Alternate protein numberPlease refer to SwissProt
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Gene number4155
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Gene nameMyelin Basic Protein
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Alternate gene nameMyelin A1 protein; Myelin membrane encephalitogenic protein
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Myelin Basic Protein (MBP). No significant cross-reactivity or interference between Myelin Basic Protein (MBP) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Myelin Basic Protein (MBP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myelin Basic Protein (MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myelin Basic Protein (MBP) level in the sample or standard.;
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaInfection immunity;Neuro science;Autoimmunity;
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WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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DescriptionBasic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer. A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbolMBP, PRG2, ENO1, HIVEP2, HIVEP1
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Short nameMyelin Protein (MBP) CLIA Kit, 96
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Labelmbp
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SpeciesHuman, Humans
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Alternative nameH. sapiens Myelin Basic Protein (MBP) CLIA reagent, 96 well plate
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Alternative techniquekits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namemyelin basic protein
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameproteoglycan 2, pro eosinophil major basic protein
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Synonyms gene name
- proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-09-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameenolase 1
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Synonyms gene
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Synonyms gene name
- enolase 1, (alpha)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Enolases
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameHIVEP zinc finger 2
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Synonyms gene name
- human immunodeficiency virus type I enhancer-binding protein 2
- human immunodeficiency virus type I enhancer binding protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-10-21
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Entrez gene record
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Pubmed identfication
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHIVEP zinc finger 1
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Synonyms gene
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Synonyms gene name
- human immunodeficiency virus type I enhancer-binding protein 1
- zinc finger protein 40
- human immunodeficiency virus type I enhancer binding protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-10-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID