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Verified reactivity
Homo sapiens (Human)
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Detection limits
78.12-20000 pg/mL
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Sensitivity limit
11,71 pg/mL
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Samples to be analyzed
Tissue homogenates, cell lysates and other biological fluids
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Assay duration
2 hours, 40min
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Verified applications
CLIA
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Assay class
Sandwich
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ELISA detection
Chemiluminescent
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Protein number
Please refer to Uniprot
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Alternate protein number
Please refer to SwissProt
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Gene number
Please refer to GenBank
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Gene name
Macrophage Scavenger Receptor 1
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Alternate gene name
CD204; MSR1; SCARA1; SR-A; PhSR1; PhSR2; Macrophage acetylated LDL receptor I and II; Scavenger receptor class A member 1
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Estimated production time
7-11 business days
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Shipping requirements
Blue ice
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Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for
8 months
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ELISA s specificity
This assay has high sensitivity and excellent specificity for detection of Macrophage Scavenger Receptor 1 (MSR1). No significant cross-reactivity or interference between Macrophage Scavenger Receptor 1 (MSR1) and analogues was observed.
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ELISA s cross reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principle
The microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Scavenger Receptor 1 (MSR1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Scavenger Receptor 1 (MSR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Scavenger Receptor 1 (MSR1) level in the sample or standard.;
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Protocol
Please see ELISA's datasheet, otherwise contact us
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Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Scavenger Receptor 1 (MSR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Scavenger Receptor 1 (MSR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main area
Signal transduction;Metabolic pathway;Infection immunity;
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Warnings
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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Notes
For research use only. Not for diagnostic procedures.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Description
A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
