Rabbit Polyclonal IgG Anti-Collagen Type II, mouse

• Catalog number
  CO20251-0.1
• Price
  Please ask
• Size
  0.1 ml

• Availability
  Please contact us to check the availability
• Immunogen
  Purified collagen type II from fetal murine cartilage
• Specificity
  Murine collagen type II 100%, murine collagen type I, III and IV
• Species reactivity
  Mouse, cross-reactivity with bovine collagen type II in ELISA
• Product presentation
  affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
• Product category
  Primary Antibody
• Antibody s raised in
  Rabbit
• Antibody s isotype
  IgG
• Antibody s clone
  polyclonal
• Product is supplied as
  purified, lyophilized
• Recommended concentration for use
  IFA ≥ 1:80, IHC(P) ≥ 1:1000, ELISA ≥ 1:200 (OD ≥ 500)
• Protein concentration
  app. 1 mg/ml
• Positive control
  Mouse cartilage
• Pretreatment
  After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Tested applications
  IHC(P), IFA, ELISA, RIA, IB/WB
• Recommended secondary reagent
  Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7401).
• Storage method
  Store at -20°C
• UniProt number
  P28481 (CO2A1_MOUSE)
• Litterature
  1. Begue-Kirn C., Smith A.J., Ruch J.V., Wozney J.M., Purchio A., Hartmann D.J., Lesot H. (1992) Effects of dentin proteins, transforming growth factor ?1 (TGFß1) and bone morphogenetic protein 2 (BMP2) on the differentiation of odontoblast in vitro. Int. J. 2. Ihanamaki T., Salminen H., Saamanen A.M., Pelliniemi L.J., Hartmann D.J., Sandberg-Lall M., Vuorio E. (2001) Age-dependent changes in the expression of matrix components in the mouse eye. Exp. Eye Res. 72, 423-431. 3. Valcourt U., Gouttenoire J., Aubert-Foucher E., Herbage D., Mallein-Gérin F. (2003) Alternative splicing of type II procollagen pre-mRNA in chondrocytes is oppositely regulated by BMP-2 and TGF- beta1. FEBS Letters 545, 115-119.
• Tips
  *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
• Other products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Gene
  Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Oryctolagus cuniculus
• Group
  Polyclonals and antibodies

• Gene target
  Collagen  
• Gene symbol
  COL2A1, P4HA2, SCARB2
• Short name
  ECM
• Technique
  Polyclonal, Rabbit, anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  IgG, IgG
• Species
  Mouse, Mouses
• Alternative name
  Collagen Type II, mouse
• Alternative technique
  polyclonals, rabbit-anti, murine, antibodies, immunoglobulins

Gene info

• Identity
  HGNC:2200
• Gene
  COL2A1
• Long gene name
  collagen type II alpha 1 chain
• Synonyms gene
  • SEDC
  • AOM
• Synonyms gene name
  • collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital)
  • arthroophthalmopathy, progressive (Stickler syndrome)
  • collagen, type II, alpha 1
  • collagen type II alpha 1
• Synonyms
  • STL1
• GenBank acession
  • X16468
• Locus
  12q13.11
• Discovery year
  1986-01-01
• Entrez gene record
  1280
• Pubmed identfication
  • 1677770
• RefSeq identity
  • NM_001844
• Classification
  • Collagens
• VEGA ID
  OTTHUMG00000149896
• Locus Specific Databases
  • LOVD - Center for Medical Genetics, Ghent, Belgium

Gene info

• Identity
  HGNC:8547
• Gene
  P4HA2
• Long gene name
  prolyl 4-hydroxylase subunit alpha 2
• Synonyms gene name
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II
  • prolyl 4-hydroxylase, alpha polypeptide II
• Synonyms
  • C-P4Halpha(II)
• Synonyms name
  • 4-PH alpha 2
  • collagen prolyl 4-hydroxylase alpha(II)
• GenBank acession
  • U90441
• Locus
  5q31.1
• Discovery year
  1999-02-23
• Entrez gene record
  8974
• Pubmed identfication
  • 9211872
  • 9149945
• RefSeq identity
  • NM_004199
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000059647

Gene info

• Identity
  HGNC:1665
• Gene
  SCARB2
• Long gene name
  scavenger receptor class B member 2
• Synonyms gene
  • CD36L2
• Synonyms gene name
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II)
• Synonyms
  • HLGP85
  • LIMPII
  • SR-BII
  • LIMP-2
• Synonyms name
  • lysosome membrane protein 2
  • lysosomal integral membrane protein II
• GenBank acession
  • D12676
• Locus
  4q21.1
• Discovery year
  1994-09-07
• Entrez gene record
  950
• Pubmed identfication
  • 1374238
• RefSeq identity
  • NM_005506
• Classification
  • Scavenger receptors
• VEGA ID
  OTTHUMG00000130099

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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