Rabbit Polyclonal IgG Anti-Collagen Type II, human
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Catalog numberCO20211-0.1
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PricePlease ask
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Size0.1 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenPurified collagen type II from human cartilage
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SpecificityHuman collagen type II, cross-reactivity with human collagen type I, IX and XI
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Species reactivityHuman
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Product presentationaffinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
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Product categoryPrimary Antibody
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Antibody s raised inRabbit
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Antibody s isotypeIgG
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Antibody s clonepolyclonal
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Product is supplied aspurified, lyophilized
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Recommended concentration for useIFA ≥ 1:80, IHC(P) ≥ 1:1000, ELISA ≥ 1:200 (OD ≥ 500)
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Protein concentrationapp. 1 mg/ml
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Positive controlHuman cartilage
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PretreatmentAfter de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
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Tested applicationsIHC(P), IFA, ELISA, RIA, IB/WB
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Recommended secondary reagentAnti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7401).
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Storage methodStore at -20°C
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UniProt numberPGo to UniProt/SwissProt2458 (CO2A1_HUMAN)
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Litterature1. Guerret S., Govignon E., Hartmann D.J., Ronfard V. (2003) Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 2003, 11, 35-45. 2. Freyria AM, Ronzière MC, Cortial D, Galois L, Hartmann D, Herbage D, Mallein-Gerin F. (2009) Comparative phenotypic analysis of articular chondrocytes cultured within type I or type II collagen scaffolds. Tissue Eng Part A. 2009, 15, 1233-1245. 3. Perrier E., Ronzière M.C.C., Bareille R., Pinzano A., Mallein-Gerin F., Freyria A.M (2011) Analysis of collagen expression during chondrogenic induction of human bone marrow mesenchymal stem cells. Biotechnol Lett. 2011 Oct;33(10):2091-2101 4. Claus S., Mayer N., Aubert-Foucher E., Chajra H, Perrier-Groult E., Lafond J., Piperno M., Damour O., Mallein-Gerin F.Cartilage-(2012) Characteristic Matrix Reconstruction by Sequential Addition of Soluble Factors During Expansion of Human Articular Chondrocytes and Their Cultivation in Collagen Sponges - Tissue Eng Part C Methods. 2012 Feb;18(2):104-112.
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GeneCollagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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GroupPolyclonals and antibodies
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolCOL2A1, P4HA2, SCARB2
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Short nameECM
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TechniquePolyclonal, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG, IgG
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SpeciesHuman, Humans
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Alternative nameCollagen Type II, human
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Alternative techniquepolyclonals, rabbit-anti, antibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene namecollagen type II alpha 1 chain
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Synonyms gene
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Synonyms gene name
- collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital)
- arthroophthalmopathy, progressive (Stickler syndrome)
- collagen, type II, alpha 1
- collagen type II alpha 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Collagens
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameprolyl 4-hydroxylase subunit alpha 2
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Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II
- prolyl 4-hydroxylase, alpha polypeptide II
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-02-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namescavenger receptor class B member 2
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Synonyms gene
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Synonyms gene name
- CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Scavenger receptors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data