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Availability
Please contact us to check the availability
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Immunogen
Purified neurofilament proteins from calf brain
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Specificity
Neurofilaments 200 kDa
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Species reactivity
Human, cattle
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Product presentation
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgM
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Antibody s clone
RNF 402
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:10 - 1:20
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Protein concentration
N/A
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Positive control
Peripheral nerves, brain
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Pretreatment
No protease pre-treatment required! Blocking of endogenous biotin and alkaline phosphatase is recommended, if avidin/biotin systems are used.
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Tested applications
IHC(P)
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Recommended secondary reagent
As secondary reagents we recommend the use of biotinylated anti-mouse IgM antibody (Art. No. BA-2020) in combination with streptavidin conjugates or ABC systems.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Osborn M., Altmannsberger M., Shaw G., Schauer A., Weber K. (1982) Various sympathetic derived human tumors differ in neurofilament expression. Use in Diagnosis of neuroblastoma, ganglioneuroblastoma and pheochromocytoma. Virchows Arch. Cell Pathol. 40; 141-156. 2. Alfonsi F., Darmon M., Forest N., and Paulin D. (1983) Intermediate sized filaments as markers of neuronal differentiation in the role of cell interaction in early neurogenesis. In: Plenum Publishing Corporation A.M. Duprat, A. Kato, and M. Weber eds. pp 157-176. 3. Osborn M., and Weber K. (1983) Biology of disease tumor diagnosis by intermediate filament typing. Lab. Invest. 48; 372-394. 4. Hacker GW, Polak JM, Springall DR, Ballesta J, Cadieux A, Gu J, Trojanowski JQ, Dahl D, Marangos PJ. (1985) Antibodies to neurofilament protein and other brain proteins reveal the innervation of peripheral organs. Immunochemistry, 82, 581-593. 5.. Osborn M., Dirk T., Käser H., Weber K., and Altmannsberger M. (1986) Immunohistochemical localization of neurofilaments and neuron-specific enolase in 29 cases of neuroblastoma. Am. J. Pathol. 122; 433-442.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The kilo Daltons subunit weight of Bioprime compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
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Properties
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
