Mouse Monoclonal IgG2a Anti-PCNA, Proliferating Cell Nuclear Antigen [PC10]
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Catalog numberPC500
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PricePlease ask
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Size5 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenRecombinant rat PCNA (Protein A expression vector pR1T2T)
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SpecificityRat, human and other vertebrate PCNA
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Species reactivityHuman, rat
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Product presentationAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG2a
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Antibody s clonePC10
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Product is supplied asready to use
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Recommended concentration for useneat
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Protein concentrationN/A
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Positive controlColon or breast carcinoma
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PretreatmentNo protease pre-treatment required
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Tested applicationsIHC(P)
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Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage methodStore at 2-8°C
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UniProt numberGo to UniProt/SwissProt
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Litterature1. Traveli S., Ku D.H., Rizzo M.G., Ottavio L., Baserga R., and Calabretta B. (1989) Structure of the human gene for the proliferating cell nuclear antigen. J. Biol. Chem. 264; 7466-7472. 2. Waseem N.H. and Lane D.P. (1990) Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar isoform. J. Cell Sci. 96; 121-129. 3. Hall P.A. and Woods A.L. (1990) Invited Review: Immunohistochemical markers of cell proliferation: achievments, problems and prospects. Cell Tissue Kinet. 23; 505-522. 4. Yu C.C.-W. and Filipe M.I. (1993) Update on proliferation-associated antibodies applicable to formalin-fixed paraffin-embedded tissue and their clinical applications. Histochem. J. 25; 843-853.
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolPCNA, PCNA-AS1
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Short nameBioprime
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TechniqueMouse monoclonal, antigen, anti-, Mouse, anti, antibody to, antibodies, antigenes, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG2a
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SpeciesMouse, Mouses
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Alternative namePCNA, Proliferating Cell Nuclear Antigen
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Alternative techniqueantigenes, murine, antibodies
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene nameproliferating cell nuclear antigen
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GenBank acession
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Locus
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Discovery year1990-01-15
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namePCNA antisense RNA 1
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Synonyms gene
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Synonyms gene name
- PCNA antisense RNA (non-protein coding)
- PCNA antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2009-08-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data