Mouse Monoclonal IgG2a Anti-EMA, Epithelial Membrane Antigen [E29]
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Catalog numberEM100
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Price:
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Size1 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenDelipidised milkfat extract
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SpecificityHuman milk fat membrane protein
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Species reactivityHuman
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Product presentationProtein A purified antibody in PBS, containing BSA and sodium azide (0.09%)
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG2a
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Antibody s cloneE29
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Product is supplied asliquid concentrate
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Recommended concentration for use1:10 - 1:50
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Protein concentration40 µg/ml
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Positive controlMammary gland epithelia, tonsil epithelium
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PretreatmentNo protease pre-treatment required. Antigen unmasking with Citrate buffer (DE000) or Unmasking Fluid G (DE007) usually will enhance staining intensity. Blocking of endogenous peroxidase or alkaline phosphatase is recommended.
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Tested applicationsIHC(C, P)
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Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage methodStore at 2-8°C
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UniProt numberGo to UniProt/SwissProt
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Litterature1. Cordell J., Richardson T.C., Pulford K.A.F., Ghosh A.K., Gatter K.C., Heydermann E., et al. (1985) Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemisty. Br. J. Cancer 52; 347-354. 2. Heydermann E., Strudley I., Powell G., Richardson T.C., Cordell J.L., and Mason D.Y. (1985) A new monoclonal antibody to epithelial membrane antigen (EMA) - E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2. Br. J. Cancer 52; 355-361. 3. Pinkus G.S., Etheridge C.L., and O'Connor E.M. (1986) Are keratin proteins a better tumor marker than epithelial membrane antigen? Am. J. Clin. Pathol. 85; 269-277. 4. Delsol G., Al Saati T., Gatter K.C., Gerdes J., Schwarting R., Caveriviere P., et al. (1988) Coexpression of epithelial membrane antigen (EMA), Ki-1, and interleukin-2 receptor by anaplastic large cell lymphomas. Am. J. Pathol. 130; 59-70.
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method. Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolETFA, MUC1
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Short nameBioprime
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TechniqueMouse monoclonal, antigen, anti-, Mouse, anti, antibody to, antibodies, antigenes, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG2a
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SpeciesMouse, Mouses
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Alternative nameEMA, Epithelial Membrane Antigen
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Alternative techniqueantigenes, murine, antibodies
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Gene info
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Identity
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Gene
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Long gene nameelectron transfer flavoprotein subunit alpha
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Synonyms gene name
- electron-transfer-flavoprotein, alpha polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemucin 1, cell surface associated
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Synonyms gene
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Synonyms gene name
- mucin 1, transmembrane
- medullary cystic kidney disease 1 (autosomal dominant)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Mucins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data