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Availability
Please contact us to check the availability
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Immunogen
Delipidised milkfat extract
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Specificity
Human milk fat membrane protein
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Species reactivity
Human
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Product presentation
Protein A purified antibody in PBS, containing BSA and sodium azide (0.09%)
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG2a
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Antibody s clone
E29
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:10 - 1:50
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Protein concentration
40 µg/ml
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Positive control
Mammary gland epithelia, tonsil epithelium
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Pretreatment
No protease pre-treatment required. Antigen unmasking with Citrate buffer (DE000) or Unmasking Fluid G (DE007) usually will enhance staining intensity. Blocking of endogenous peroxidase or alkaline phosphatase is recommended.
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Tested applications
IHC(C, P)
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Cordell J., Richardson T.C., Pulford K.A.F., Ghosh A.K., Gatter K.C., Heydermann E., et al. (1985) Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemisty. Br. J. Cancer 52; 347-354. 2. Heydermann E., Strudley I., Powell G., Richardson T.C., Cordell J.L., and Mason D.Y. (1985) A new monoclonal antibody to epithelial membrane antigen (EMA) - E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2. Br. J. Cancer 52; 355-361. 3. Pinkus G.S., Etheridge C.L., and O'Connor E.M. (1986) Are keratin proteins a better tumor marker than epithelial membrane antigen? Am. J. Clin. Pathol. 85; 269-277. 4. Delsol G., Al Saati T., Gatter K.C., Gerdes J., Schwarting R., Caveriviere P., et al. (1988) Coexpression of epithelial membrane antigen (EMA), Ki-1, and interleukin-2 receptor by anaplastic large cell lymphomas. Am. J. Pathol. 130; 59-70.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method. Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
