Mouse Monoclonal IgG2a Anti-CML (N-Epsilon)-Carboxymethyl-Lysine : Biotin conjugate [NF-1G]
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Catalog numberCML024BIO
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PricePlease ask
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Size50 µg
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AvailabilityPlease contact us to check the availability
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ImmunogenN-Epsilon-(Carboxymethyl)-Lysine (CML)-HSA
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Species reactivitynot relevant
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Product presentationProtein G affinity purified antibody from ascites in stabilized buffer, containing 50% Block Ace (Casein-containing solution, Dainippon Co.) and 0.1% ProClin (Rohm & Haas) as a preservative
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG2a
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Antibody s cloneNF-1G
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Product is supplied asliquid concentrate
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Recommended concentration for useELISA 0.1-0.5 µg/ml; IHC 2 µg/ml
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Protein concentration0.25 mg/ml
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Tested applicationsIHC, ELISA
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Recommended secondary reagentStreptavidin:HRPO conjugate (Art. No. ZU054) or alkaline phosphatase conjugate (Art.-Nr. ZU051) in combination with a suitable substrate.
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Storage methodStore at -20°C
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UniProt numberGo to UniProt/SwissProt
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Litterature1. Horiuchi S., Araki N., and Morino Y. (1991) Immunological approach to characterize advanced glycation end products of the Maillard reaction: Evidence for the presence of a common stucture. J. Biol. Chem. 266; 7329-7332. 2. Dunn J.A., Patrick J.S., Thorpe S.R., Baynes J.W. (1989): Oxidation of glycated proteins: age-dependent accumulation of N-epsilon-(carboxymethyl) lysine in lens proteins. Biochemistry. 28: 9464-9468. 3. Fu M.X., Requena J.R., Jenkins A.J., Lions T.J., Baynes J.W., Thorpe S.R. (1996): The advanced glycation end product, N-epsilon-(carboxymethyl) lysine, is a product of both lipid peroxidation and glycoxidation reactions. J.Biol.Chem.271: 9982-9986
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TipsGentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolNFE4, NFE2, NEFH, NFKB1, INA, NFASC, NFATC1, NFATC2, NFYC, BCR
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Short nameAGE
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TechniqueMouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG2a
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LabelBiotin
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SpeciesMouse, Mouses
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Alternative nameCML (N-Epsilon)-Carboxymethyl-Lysine : Biotin conjugate
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene namenuclear factor, erythroid 4
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Synonyms
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Locus
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Discovery year2013-08-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear factor, erythroid 2
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Synonyms gene name
- nuclear factor (erythroid-derived 2), 45kD
- nuclear factor (erythroid-derived 2), 45kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-11-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Basic leucine zipper proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameneurofilament heavy chain
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Synonyms gene name
- neurofilament, heavy polypeptide 200kDa
- neurofilament heavy polypeptide
- neurofilament heavy
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Synonyms
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Locus
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Discovery year1988-08-31
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Entrez gene record
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RefSeq identity
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Classification
- Intermediate filaments Type IV
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namenuclear factor kappa B subunit 1
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Synonyms gene name
- nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-11-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- NF-kappa B complex subunits
- Ankyrin repeat domain containing
- IPT domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameinternexin neuronal intermediate filament protein alpha
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Synonyms gene
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Synonyms gene name
- internexin neuronal intermediate filament protein, alpha
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-03-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Intermediate filaments Type IV
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameneurofascin
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Synonyms gene name
- neurofascin homolog (chicken)
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-11-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ig-like cell adhesion molecule family
- I-set domain containing
- Fibronectin type III domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear factor of activated T cells 1
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Synonyms gene name
- nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
- nuclear factor of activated T-cells 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-11-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nuclear factors of activated T-cells
- IPT domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear factor of activated T cells 2
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Synonyms gene name
- nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
- nuclear factor of activated T-cells 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-11-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- IPT domain containing
- Nuclear factors of activated T-cells
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear transcription factor Y subunit gamma
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Synonyms gene name
- nuclear transcription factor Y, gamma
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-10-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameBCR activator of RhoGEF and GTPase
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Synonyms gene
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Synonyms gene name
- breakpoint cluster region
- BCR, RhoGEF and GTPase activating protein
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Synonyms
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2 domain containing
- My-T-BRC complex
- Dbl family Rho GEFs
- Pleckstrin homology domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
