Mouse Monoclonal IgG1 Anti-TTF-1 (Thyroid Transcription Factor 1), Homeobox protein Nkx-2.1 [8G7G3/1]
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Catalog numberTT500
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PricePlease ask
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Size5 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenRecombinant rat TTF-1
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SpecificityThyroid Transcription Factor-1 (TTF-1), 38.5 kDa nuclear protein
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Species reactivityRat, Mouse, Human
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Product presentationAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG1
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Antibody s clone8G7G3/1
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Product is supplied asready to use
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Recommended concentration for useneat
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Protein concentrationN/A
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Positive controlThyroid or Lung
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PretreatmentFor pre-treatment we recommend Biologo's Unmasking Fluid G on glycol basis (Art. No. DE007) at high temperature.
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Tested applicationsIHC(P)
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Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage methodStore at 2-8°C
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UniProt numberP23441 (NKX21_RAT)
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Litterature1. Holzinger A., Dingle S., Bejarano P.A., Miller M.A., Weaver T.E., DiLauro R., Whitsett J.A. (1996) Monoclonal antibody to thyroid transcription factor-1: production, characterization, and usefulness in tumor diagnosis. Hybridoma 15(1); 49-53. 2. Bejarano P.A., Baughman R.P., Biddinger P.W., Miller M.A., Fenoglio-Preiser C., al-Kafaji B., Di Lauro R., Whitsett J.A. (1996)Surfactant proteins and thyroid transcription factor-1 in pulmonary and breast carcinomas. Modern Pathology 9(4); 445-452. 3. Bohinski. R.J., Bejarano P.A., Balko G., Warnick R.E., and Whitsett J.A. (1998) Determination of lung as the primary site of cerebral metastatic adenocarcinomas using monoclonal antibody to thyroid transcription factor-1. J. Neurooncol. 40(3); 227-231. 4. Ordonez N.G. (2000) Thyroid transcription factor-1 is a marker of lung and thyroid carcinomas. Adv. Anat. Pathol. 7(2);123-127. 5. Ikeda K., Clark J.C., Shaw-White J.R. Stahlman M.T., Boutell C.J., and Whitsett J.A. (1995) Gene structure and expression of human thyroid transcription factor-1 in respiratory epithelial cells. J. Biol. Chem. 270(14; 8108-8114
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolNKX2-1, TTF1, RHOH, FOXE1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1
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Short nameBioprime
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TechniqueMouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG1
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SpeciesMouse, Mouses
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Alternative nameTTF-1 (Thyroid Transcription Factor 1), Homeobox protein Nkx-2.1
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Alternative techniquemurine, antibodies
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Tissuethyroid
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Gene info
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Identity
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Gene
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Long gene nameNK2 homeobox 1
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Synonyms gene
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Synonyms gene name
- benign chorea
- thyroid transcription factor 1
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Synonyms
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Locus
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Discovery year1992-06-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- NKL subclass homeoboxes and pseudogenes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametranscription termination factor 1
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Synonyms gene name
- transcription termination factor, RNA polymerase I
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GenBank acession
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Locus
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Discovery year1996-05-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Myb/SANT domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameras homolog family member H
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Synonyms gene
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Synonyms gene name
- ras homolog gene family, member H
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-04-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Rho family GTPases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameforkhead box E1
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Synonyms gene
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Synonyms gene name
- forkhead box E2
- forkhead box E1 (thyroid transcription factor 2)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-02-14
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Entrez gene record
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Pubmed identfication
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Classification
- Forkhead boxes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
