Mouse Monoclonal IgG1 Anti-PAP, Prostate Acid Phosphatase (PSAP) [PASE/4LJ]

  • Catalog number
    PA101
  • Price
    Please ask
  • Size
    1 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Purified human Prostate Acid Phosphatase from seminal plasma
  • Specificity
    Human PAP, 48 kDa subunit, no cross reactivity with PSA
  • Species reactivity
    Human
  • Product presentation
    Purified antibody in PBS containing BSA and 15mM NaN3
  • Product category
    Primary Antibody
  • Antibody s raised in
    Mouse
  • Antibody s isotype
    IgG1
  • Antibody s clone
    PASE/4LJ
  • Product is supplied as
    liquid concentrate
  • Recommended concentration for use
    1:25-1:50
  • Protein concentration
    N/A
  • Positive control
    Prostate carcinoma
  • Pretreatment
    HIER (heat induced epitope retrieval)
  • Tested applications
    IHC (P)
  • Recommended secondary reagent
    We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
  • Storage method
    Store at 2-8°C
  • UniProt number
    P153Go to UniProt/SwissProt9 (PPAP_HUMAN)
  • Litterature
    1. Sharief FS, Lee H, Leuderman MM, Lundwall A, Deaven LL, Lee CL, Li SS (1989) Human prostatic acid phosphatase: cDNA cloning, gene mapping and protein sequence homology with lysosomal acid phosphatase. Biochem Biophys Res Commun. Apr 14;160(1):79-86. 2. Veeramani S, Yuan TC, Chen SJ, Lin FF, Petersen JE, Shaheduzzaman S, Srivastava S, MacDonald RG, Lin MF (2005) Cellular prostatic acid phosphatase: a protein tyrosine phosphatase involved in androgen-independent proliferation of prostate cancer. Endocr Relat Cancer. Dec;12(4):805-22. 3. Graddis TJ, McMahan CJ, Tamman J, Page KJ, Trager JB (2011) Prostatic acid phosphatase expression in human tissues. Int J Clin Exp Pathol. Mar;4(3):295-306. Epub 2011 Mar 22. 4. Queisser A., Hagedorn S.A., Braun M., Vogel W., Duensing S., Perner S. (2015) Comparison of different prostatic markers in lymph node and distant metastases of prostate cancer. ModernPathology 28, 138–145.
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    PAPOLA, ACP3, RP9, PSAP, MRPS30, PDAP1, MTCH1, TUSC2, PLPP2, PLPP3
  • Short name
    BioPrime
  • Technique
    Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Isotype
    IgG1
  • Species
    Mouse, Mouses
  • Alternative name
    PAP, Prostate Acid Phosphatase (PSAP)
  • Alternative technique
    murine, antibodies
  • Tissue
    prostate
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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