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Availability
Please contact us to check the availability
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Immunogen
Recombinant protein corresponding to the C-terminus of human MSH2
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Specificity
C-terminus of human MSH2 protein
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Species reactivity
Human, other species not tested
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Product presentation
Protein G purified antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05 % sodium azide**
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1
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Antibody s clone
FE11
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1/500
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Protein concentration
1 mg/ml
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Positive control
HTC116 or SW-480 cells or colon tissue
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Pretreatment
Paraffin slides must be pretreated with HIER (Unmasking fluid C, Art.-Nr. DE000) or Proteinase K.
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Tested applications
IHC (C,P) (2 µg/ml), IB (0,2 µg/ml), IP (1 µg/ml), IF, WB (1 µg/ml)
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Recommended secondary reagent
Anti-Mouse IgG-conjugates, biotinylated (Art.-Nr. BA-2000 or BA-2001), HRPO (Art.-Nr. PI-2000), FITC (Art.-Nr. FI-2000 or FI-2001) or VECTOR ImmPress anti-mouse Ig polymer detection kit (Art.-Nr. MP-7402)
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Storage method
Store at 2-8°C
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UniProt number
P43246.1
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Litterature
1. Leach FS, Polyak K, Burrell M, Johnson KA, Hill D, Dunlop MG, Wyllie AH, Peltomaki P, de la Chapelle A, Hamilton SR, Kinzler KW, Vogelstein B. Expression of the human mismatch repair gene hMSH2 in normal and neoplastic tissues. Cancer Res. 1996 Jan 15;56(2):235-40. 2. Thibodeau SN, French AJ, Roche PC, Cunningham JM, Tester DJ, Lindor NM, Moslein G, Baker SM, Liskay RM, Burgart LJ, Honchel R, Halling KC Altered expression of hMSH2 and hMLH1 in tumors with microsatellite instability and genetic alterations in mismatch repair genes. Cancer Res. 1996 Nov 1;56(21):4836-40.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
MSH2-OT1, MSH2
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Short name
Research
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Technique
Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG1
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Species
Mouse, Mouses
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Alternative name
MSH2, Mismatch Repair Protein 2 (FE11)
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Alternative technique
murine, antibodies
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Alternative to gene target
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli), COCA1 and FCC1 and HNPCC and HNPCC1 and LCFS2, MSH2 and IDBG-50900 and ENSG00000095002 and 4436, ADP binding, nuclei, Msh2 and IDBG-201483 and ENSMUSG00000024151 and 17685, MSH2 and IDBG-633627 and ENSBTAG00000002742 and 533115
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