Rabbit Anti Human EGFR Polyclonal Antibody - FITC Conjugated
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Catalog number
OACA01698
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Price
Please ask
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Size
100 ug
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Recognized antigen
EGFR
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Product type
Antibody
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Gene name
EGFR
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Long name
Antibody against EGFR
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Storage
For shorter time periods the EGFR Antibodies may be kept refrigerated at +4 degrees Celsius. For extended periods of time keep frozen at -20 °C. Avoid freezing and thawing the lysate repeatedly as these cycles will cause denaturation and/or disruption of the protein chains and peptide bonds of the EGFR Antibodies which might cause change or loss of the desired properties.
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Tips
Small amounts of the EGFR antibody may get entrapped on the walls or lid of the vial as a result of shaking during transportation or handling. Prior to use, cetrifuge the vial to ensure that all of its content is collected on the bottom. In order to avoid cycles of freezing and thawing we recommend to plan and prepare aliquots according to the experiment demand and defrost only the amount which wil be needed for immediate use.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
If you buy Antibodies supplied by aviva they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This aviva Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aviva FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by aviva. Rabbit antibodies are very stable and can be stored for several days at room temperature. aviva adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
EGFR-AS1, EGILA, EGFR, RHBDF1
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Short name
Anti-EGFR
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Technique
anti human, Polyclonal, Antibody, Rabbit, anti, FITC, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Label
FITC
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Species
Human, Humans
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Alternative name
EGFR Antibodies
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Alternative technique
polyclonals, antibodies, rabbit-anti, fluorescine
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Alternative to gene target
epidermal growth factor receptor, ERBB and ERBB1 and HER1 and mENA and PIG61, EGFR and IDBG-16933 and ENSG00000146648 and 1956, transferase activity, nuclei, Egfr and IDBG-151194 and ENSMUSG00000020122 and 13649, EGFR and IDBG-644035 and ENSBTAG00000011628 and 407217
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Gene info
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Identity
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Gene
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Long gene name
EGFR antisense RNA 1
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Locus
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Discovery year
2012-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
EGFR interacting lncRNA
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Synonyms
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Locus
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Discovery year
2019-09-27
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Pubmed identfication
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Classification
- Long non-coding RNAs with non-systematic symbols
Gene info
Gene info
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Identity
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Gene
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Long gene name
rhomboid 5 homolog 1
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 8
- rhomboid family 1 (Drosophila)
- rhomboid 5 homolog 1 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year
2003-04-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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