MCF-7 Cell Nuclear Extract Lysate

• Catalog number
  OCRA00107
• Price
  Please ask
• Size
  one vial

• Description
  Our lysate from MCF-7 Cell Nuclear Extract is a high quality product which can readily be used in various applications such as Western Blot (WB) ELISA, EIA, IFA, etc. The MCF-7 Cell Nuclear Extract Lysate will be an excellent control for these applications.
• Storage
  For shorter time periods the MCF-7 Cell Nuclear Extract Lysate may be kept refrigerated at +4 degrees Celsius. For extended periods of time keep frozen at -20 °C. Avoid freezing and thawing the lysate repeatedly as these cycles will cause denaturation and/or disruption of the protein chains and peptide bonds which might cause change or loss of the desired properties.
• Tips
  Small amounts of the MCF-7Nuclear Extract Lysate may get entrapped on the walls or lid of the vial as a result of shaking during transportation or handling. Prior to use, cetrifuge the vial to ensure that all of its content is collected on the bottom. In order to avoid cycles of freezing and thawing we recommend to plan and prepare aliquots according to the experiment demand and defrost only the amount which wil be needed for immediate use.
• Additional description
  For cells, cell lines and tissues in culture till half confluency. Pure extracts used in broths or as control for cell culture and Western Blot.

• Gene target
  MCF-7   Lysate  
• Gene symbol
  PIRC34, PIRC33, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7, RNU6-7, PIRC30, PIRC31, PIRC29
• Short name
  MCF-7Nuclear Extract Lysate
• Technique
  extract, extracts
• Alternative name
  Lysate from MCF-7 Cell Nuclear Extract
• Alternative technique
  extrats
• Tissue
  cell, lysate
• Virus
  lysate

Gene info

• Identity
  HGNC:37538
• Gene
  PIRC34
• Long gene name
  piwi-interacting RNA cluster 34
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313890
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37537
• Gene
  PIRC33
• Long gene name
  piwi-interacting RNA cluster 33
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313854
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37536
• Gene
  PIRC32
• Long gene name
  piwi-interacting RNA cluster 32
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313811
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:32995
• Gene
  SNORD114-7
• Long gene name
  small nucleolar RNA, C/D box 114-7
• Synonyms
  • 14q(II-7)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767583
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003199.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5633
• Gene
  IGHV3OR15-7
• Long gene name
  immunoglobulin heavy variable 3/OR15-7 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 3/OR15-7
  • immunoglobulin heavy variable 3/OR15-7 pseudogene
• Synonyms
  • IGHV3/OR15-7
• GenBank acession
  • Z29597
• Locus
  15q11.2
• Discovery year
  2000-04-17
• Entrez gene record
  28318
• Classification
  • Immunoglobulin heavy (IGH) orphons
• VEGA ID
  OTTHUMG00000171835

Gene info

• Identity
  HGNC:33026
• Gene
  SNORD115-7
• Long gene name
  small nucleolar RNA, C/D box 115-7
• Synonyms
  • HBII-52-7
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033444
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003299.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:34284
• Gene
  RNU6-7
• Long gene name
  RNA, U6 small nuclear 7
• Synonyms
  • U6-7
• Locus
  14q12
• Discovery year
  2008-06-10
• Entrez gene record
  101954275
• Pubmed identfication
  • 12711679
• RefSeq identity
  • NR_104084
• Classification
  • Small nuclear RNAs

Gene info

• Identity
  HGNC:37534
• Gene
  PIRC30
• Long gene name
  piwi-interacting RNA cluster 30
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313853
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37535
• Gene
  PIRC31
• Long gene name
  piwi-interacting RNA cluster 31
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313787
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37533
• Gene
  PIRC29
• Long gene name
  piwi-interacting RNA cluster 29
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313927
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

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