293T Cell Lysate

Description

Our lysate from 293T Cell is a high quality product which can readily be used in various applications such as Western Blot (WB) ELISA, EIA, IFA, etc. The 293T Cell Lysate will be an excellent control for these applications.
Storage

For shorter time periods the 293T Cell Lysate may be kept refrigerated at +4 degrees Celsius. For extended periods of time keep frozen at -20 °C. Avoid freezing and thawing the lysate repeatedly as these cycles will cause denaturation and/or disruption of the protein chains and peptide bonds which might cause change or loss of the desired properties.
Tips

Small amounts of the 293T Lysate may get entrapped on the walls or lid of the vial as a result of shaking during transportation or handling. Prior to use, cetrifuge the vial to ensure that all of its content is collected on the bottom. In order to avoid cycles of freezing and thawing we recommend to plan and prepare aliquots according to the experiment demand and defrost only the amount which wil be needed for immediate use.
Additional description

For cells, cell lines and tissues in culture till half confluency.

Gene target

Lysate
Short name

293T Lysate
Technique

CV40 transformed cell line or 293tsA1609neo from human kidney 293 cells delivered as 1.
Alternative name

Lysate from 293T Cell
Tissue

cell, lysate
Virus

lysate

Name

Transformation, Bacterial
Concept

Scope note: The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.
Tree numbers
- E05.393.350.810.500
Qualifiers

immunology, physiology, ethics, drug effects, radiation effects, genetics