Akt1 (Phospho-Thr450) Antibody

Applications

WB IHC ELISA
Source

Rabbit
Reactivity

H, M, R
MW kDa

55 kDa
Target Modification

Phospho
Modification Sites

H:T450 M:T450 R:T450
Immunogen

The antiserum was produced against synthesized peptide derived from human Akt1 around the phosphorylation site of Thr450.
Immunogen Range

416-465
Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity

Akt1 (Phospho-Thr450) Antibody detects endogenous levels of Akt1 only when phosphorylated at Thr450.
Gene ID

207
Swiss Prot No

P31749
OMIM No

164730
Unigene No

Hs.525622
NCBI Gene Symbol

AKT1
Synonyms

AKT; AKT1 kinase; C-AKT; kinase Akt1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-alpha serine/threonine kinase; RAC-PK-alpha
Storage Stability

Stable at -20°C for at least 1 year.
Physical Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality

Polyclonal
Concentration

1 mg/ml
Properties

If you buy Antibodies supplied by Assay Biotech they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation

anticorps

Gene target

Akt1 Phospho-Thr450
Short name

Akt1 (Phospho-Thr450) Antibody
Technique

Antibody, antibodies against human proteins, antibodies for
Alternative name

v-akt murine thymoma viral oncogene homolog 1 (phosphorilated-Thr450) (antibody to-)
Alternative technique

antibodies
Alternative to gene target

v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data