anti-5Methylcytosine antibody mouse, IgM (clone 5MC-CD), FITC-conjugated,IF/ICC, Azide and carrier free
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Catalog number
51-005
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Price
Please ask
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Size
50ug
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. AS ONE INTERNATIONAL supplies azide free antibodies on request. A carrier can hold protected the subject it is supporting for the period needed by its application as stated by AS ONE INTERNATIONAL.
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Properties
If you buy Antibodies supplied by AS ONE INTERNATIONAL they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This AS ONE INTERNATIONAL Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. AS ONE INTERNATIONAL FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
M6PR, MIA, NOD2, CTLA4
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Short name
anti-5Methylcytosine antibody mouse, IgM (clone 5MC-CD), FITC-conjugated IF/ICC, free
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Technique
Antibody, anti-, Mouse, anti, FITC, IgM, IF, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, IgMs, mouses
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Host
mouse
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Isotype
IgM, IgM
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
Antibody to5Methylcytosine (antibody to-) mouse, Immunoglobulin M (clonality 5MC-CD), fluorecein-coupled,IF/ICC, Azide and carrier free
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Alternative technique
antibodies, murine, fluorescine, immunoglobulin-m
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Clone name
5MC-CD
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Gene info
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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