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Available ordering format
Lyophilized
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Immunogen
V5 tag sequence GKPIPNPLLGLDST
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8°C. Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Verified applications
Western blot (WB)
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Connected products
AS15 2964 | V5, ALP conjugated (rabbit antibody)AS15 2966 | V5, DyLight® 488 conjugated (rabbit antibody)AS15 2967 | V5, DyLight® 550 conjugated (rabbit antibody)AS15 2968 | V5, DyLight® 650 conjugated (rabbit antibody)AS15 2969 | V5, SureLight® R-Phycoerythrin conjugated (rabbit antibody)AS15 3031 | V5 (mouse antibody)other tag antibodies
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Recommended dilutions for use
to be determined by end user
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
to be added when available, antibody released in August 2015
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Scientific context
Rabbit anti-V5 is a primary antibody which binds to V5 and is directly conjugated to biotin. This is of advantage to shorten assay time by no need to use a secondary antibody to V5.
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Notes
Antibody is provided in: 100 mM Sodium Phosphate pH 7.4, 100 mM Sodium Chloride, 50 mM Sucrose, 0.1% BSA, and 2 mM Sodium Azide.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated