V-ATPase, H, vacuolar H+-ATPase subunit H

• Catalog number
  AS09 499
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Inquire
• Immunogen
  KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana V-ATPase subunit H Q9LX65, At3g42050
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
• Verified applications
  ELISA (ELISA), western blot (WB)
• Connected products
  collection of antibodies to vacuolar proteins AS09 466 | anti-V-ATPase subunit a | vacuolar H+ ATPase, subunit aAS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPaseAS09 503 | anti-V-ATPase, B | vacuolar ATP synthase subunit betaAS09 468 | anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa) ASS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit DAS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPasePlant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
• Molecular weight expected аpparent
  50 kDa
• Verified reactivity
  Arabidopsis thaliana
• Possible reactivity
  dicots including: Spinacia oleracea, monocots including: Hordeum vulgare, Oryza sativa, trees: Picea sitchensis, Populus balsamifera subsp. trichocarpa, algae: Chlamydomonas reinhardtii
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
• References
  to be added when available
• Scientific context
  V-ATPase is an enzyme responsible for acidification of intracellular compartments. Subunit H is part of the peripheral V1 complex. Alternative name: vacuolar proton pump subunit H, vacuolar H(+)-ATPase subunit H
• Notes
  0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol of isolation of vacuolar membranes can be found here.
• Protein number
  Q9LX65
• TAIR number
  AT3G42050
• Hyperlink

• Gene target
  V-ATPase   vacuolar   ATPase   subunit  
• Gene symbol
  ATP6V1H, ATP6V0B, ATP6V1D
• Short name
  V-ATPase, H, vacuolar H+-ATPase subunit H
• Alternative name
  V-ATPase, H, vacuolar H+-ATPase functionnal sequence H

Gene info

• Identity
  HGNC:18303
• Gene
  ATP6V1H
• Long gene name
  ATPase H+ transporting V1 subunit H
• Synonyms gene name
  • ATPase, H+ transporting, lysosomal 50/57kD, V1 subunit H
  • ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
• Synonyms
  • CGI-11
  • SFD
  • VMA13
  • SFDalpha
  • SFDbeta
• Synonyms name
  • vacuolar ATP synthase subunit H
  • V-ATPase subunit H
• GenBank acession
  • AF132945
• Locus
  8q11.23
• Discovery year
  2002-05-09
• Entrez gene record
  51606
• Pubmed identfication
  • 9620685
  • 10810093
• RefSeq identity
  • NM_015941
• Classification
  • V-type ATPase subunits
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000164231

Gene info

• Identity
  HGNC:861
• Gene
  ATP6V0B
• Long gene name
  ATPase H+ transporting V0 subunit b
• Synonyms gene
  • ATP6F
• Synonyms gene name
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump) 21kD
  • ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c''
  • ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b
• Synonyms
  • VMA16
  • HATPL
• Synonyms name
  • V-ATPase subunit b
• GenBank acession
  • BC000423
• Locus
  1p34.1
• Discovery year
  1997-08-18
• Entrez gene record
  533
• Pubmed identfication
  • 9653649
• RefSeq identity
  • NM_004047
• Classification
  • V-type ATPase subunits
• VEGA ID
  OTTHUMG00000008298

Gene info

• Identity
  HGNC:13527
• Gene
  ATP6V1D
• Long gene name
  ATPase H+ transporting V1 subunit D
• Synonyms gene
  • ATP6M
• Synonyms gene name
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump)
  • ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
• Synonyms
  • VATD
  • VMA8
• Synonyms name
  • V-ATPase subunit D
• GenBank acession
  • AF145316
• Locus
  14q23.3
• Discovery year
  2001-08-01
• Entrez gene record
  51382
• Pubmed identfication
  • 9442887
• RefSeq identity
  • NM_015994
• Classification
  • V-type ATPase subunits
• VEGA ID
  OTTHUMG00000171244

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