V-ATPase, D, vacuolar H+-ATPase subunit D
-
Catalog numberAS09 497
-
PricePlease ask
-
Size100 µl
-
-
Available ordering formatInquire
-
ImmunogenKLH-conjugated synthetic peptide conserved in Arabidopsis thaliana V-ATPase subunit D Q9XGM1, At3g58730
-
Raised inRabbit
-
ClonalityPolyclonal
-
ClonePolyclonal
-
PurificationSerum
-
How to reconstituteSee included datasheet or contact our support service
-
Storage conditionstore at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
-
Verified applicationsELISA (ELISA), western blot (WB)
-
Connected productscollection of antibodies to vacuolar proteins AS09 466 | anti-V-ATPase subunit a | vacuolar H+ ATPase, subunit aAS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPaseAS09 503 | anti-V-ATPase, B | vacuolar ATP synthase subunit betaAS09 468 | anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa) ASS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit DAS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPaseAS09 499 | anti-V-ATPase subunit H |V-type proton ATPase subunit HPlant protein extraction bufferSecondary antibodies
-
Recommended dilutions for use1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
-
Molecular weight expected аpparent29 | 29 kDa
-
Verified reactivityArabidopsis thaliana
-
Possible reactivitydicots including: Sorghum bicolor, monocots including: Zea mays
-
No reactivityno confirmed exceptions from predicted reactivity known in the moment
-
Supplementary informationProtein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
-
Referencesto be added when avaialble
-
Scientific contextV-ATPase is an enzyme responsible for acidification of intracellular compartments. Subunit D is the peripheral subunit of V1 complex. Alternative name: Vacuolar proton pump subunit D, vacuolar H(+)-ATPase subunit D
-
Notes0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol of isolation of vacuolar membranes can be found here.
-
Protein numberQ9XGM1
-
TAIR numberAT3G58730
-
Gene target
-
Gene symbolATP6V1D, ATP6V0D1
-
Short nameV-ATPase, D, vacuolar H+-ATPase subunit D
-
Alternative nameV-ATPase, D, vacuolar H+-ATPase functionnal sequence D
-
Gene info
-
Identity
-
Gene
-
Long gene nameATPase H+ transporting V1 subunit D
-
Synonyms gene
-
Synonyms gene name
- ATPase, H+ transporting, lysosomal (vacuolar proton pump)
- ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-08-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- V-type ATPase subunits
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameATPase H+ transporting V0 subunit d1
-
Synonyms gene
-
Synonyms gene name
- ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D
- ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d isoform 1
- ATPase, H+ transporting, lysosomal 38kDa, V0 subunit D1
- ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2000-11-08
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- V-type ATPase subunits
-
VEGA ID