UBQ11, Ubiquitin, affinity purified antibody

• Catalog number
  AS08 307A
• Price
  Please ask
• Size
  50 µg

• Available ordering format
  Lyophilized
• Immunogen
  recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag; TAIR: At4g05050
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified serum
• How to reconstitute
  For reconstitution add 50 µl of sterile water
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  Western blot (WB)
• Connected products
  AS08 307 | UBQ11 | Ubiquitin, rabbit antibody, provided in a serum formatAS10 307S | UBQ11 | Ubiquitin protein | positive control
• Recommended dilutions for use
  1: 10 000 with standard ECL (WB)
• Molecular weight expected аpparent
  8.5 kDa
• Verified reactivity
  Arabidopsis thaliana, Nicotiana benthamina, Oryza sativa
• Possible reactivity
  dicots including: Nicotiana tabacum, Glycine max, Pisum sativum, monocots including: Hordeum vulgare, Oryza sativa, Zea mays
• No reactivity
  algae
• Supplementary information
  antibody can be used to check qubiquitination status in the whole plant extracts.
• References
  to be added when available, antibody released in November 2013.
• Scientific context
  Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).
• Notes
  None
• Protein number
  Refer to NCBI
• TAIR number
  AT4G05050
• Hyperlink
• Test
  A high affinity purification column was use to purify UBQ11, Ubiquitin, antibody by agisera by chromatographic size exclusion.
• Description
  1 of protein A or G purified by agisera will give more specificity than crude serum.
• Properties
  If you buy Antibodies supplied by agisera they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  UBQ11   Ubiquitin  
• Short name
  UBQ11, Ubiquitin, antibody
• Technique
  affinity, Antibody, purified, antibodies against human proteins, antibodies for, purifications
• Alternative name
  UBQ11, Ubiquitin, protein enriched (antibody to-)
• Alternative technique
  pure, antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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