-
Available ordering format
Lyophilized
-
Immunogen
Recombinant fragment of CP protein as described in Gorovits et al. (2013).
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Serum
-
How to reconstitute
For reconstitution add 150 µl of sterile water.
-
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applications
western blot (WB), immunofluorescence (IF)
-
Connected products
AS15 3055 | anti-TYLCV C1 | Tomato yellow leaf curl virus coat protein C1, rabbit antibodiesAS15 3056 | anti-TYLCV C2 | Tomato yellow leaf curl virus coat protein C2, rabbit antibodiesAS15 3057 | anti-TYLCV C3 | Tomato yellow leaf curl virus coat protein C3, rabbit antibodiesAS15 3058 | anti-TYLCV C4 | Tomato yellow leaf curl virus coat protein C4, rabbit antibodiesAS15 3059 | anti-TYLCV V2 | Tomato yellow leaf curl virus coat protein V2, rabbit antibodiesPlant and algal protein extraction buffer Secondary antibodies
-
Recommended dilutions for use
1 : 500 (WB), 1: 1000 - 1: 2000 (IF)
-
Molecular weight expected аpparent
30 kDa
-
Verified reactivity
Tomato yellow leaf curl virus coat 30 kDa
-
Possible reactivity
Tomato yellow leaf curl virus coat 30 kDa
-
No reactivity
no confirmed exceptions from predicted reactivity are currently known
-
Supplementary information
This antibody is detecting recombinant TYLCV CP protein.
-
References
Gorovits et al. (2013). Progressive aggregation of Tomato yellow leaf curl virus coat protein in systemically infected tomato plants, susceptible and resistant to the virus. Virus Res. 2013 Jan;171(1):33-43. doi: 10.1016/j.virusres.2012.09.017. Epub 2012 Oct 22.
-
Scientific context
TYLCV CP (Tomato yellow leaf curl virus coat protein 30 kDa) accumulates in tomato leaves during infection. This protein was detected in the phloem of associated cells. TYLCV virus attacts tomato cultures worldwide and is transmitted by the white flye Bemisia tabaci.
-
Notes
To detect CP protein in western blot, extract has to be concentrated 10x.
-
Protein number
Refer to NCBI
-
TAIR number
Refer to NCBI
-
-
Description
The kilo Daltons subunit weight of TYLCV CP, Tomato leaf curl virus coat protein 30 compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein