Toc75, Protein TOC75-3, chloroplastic, POTRA domain 3

• Catalog number
  AS08 351
• Price
  Please ask
• Size
  200 µl

• Available ordering format
  Lyophilized
• Immunogen
  psTOC75; Predicted POTRA Domain #3; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 200 µl of sterile water
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  Western blot (WB), flow cytometry (FACS), immunolocalization (IL)
• Connected products
  AS08 345 | anti- Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1 rabbit antibodiesPlant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 2000 - 1: 25 000, 1: 100 000 (WB), 1: 500 (FACS). 1: 100 (IL)
• Molecular weight expected аpparent
  88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
• Verified reactivity
  Arabidopsis thaliana, Nicotiana tabacum, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
• Possible reactivity
  Ricinus communis, Vitis vinifera, Populus trichocarpa
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction
• References
  Sasaki and Yamamoto (2015). Arabidopsis LAZY1 is a peripheral membrane protein of which the carboxy-terminal fragment potentially interacts with microtubules. Copyright © 2015 The Japanese Society for Plant Cell and Molecular Biology, Plant Biotechnology, 32, 1-6 (2015) DOI: 10.5511/plantbiotechnology.15.0116a Hsueh et al. (2014). The chloroplast outer envelope protein P39 in Arabidopsis thaliana belongs to the Omp85 protein family. Proteins. 2014 Nov 17. doi: 10.1002/prot.24725. Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
• Scientific context
  OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303); FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
• Notes
  None
• Protein number
  Q43715
• TAIR number
  AT3G46740
• Hyperlink

• Gene target
  Toc75   Protein   TOC75-3   chloroplastic   POTRA   domain  
• Gene symbol
  MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12, MIR9-3, MIR941-3, PIRC15
• Short name
  Toc75, Protein TOC75-3, chloroplastic, POTRA domain 3
• Alternative name
  Toc75, Protein TOC75-3, chloroplastic, POTRA domain 3

Gene info

• Identity
  HGNC:35295
• Gene
  MIR1302-3
• Long gene name
  microRNA 1302-3
• Synonyms gene
  • MIRN1302-3
• Synonyms
  • hsa-mir-1302-3
• Locus
  2q14.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302128
• RefSeq identity
  • NR_031632
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:33675
• Gene
  MIR509-3
• Long gene name
  microRNA 509-3
• Synonyms gene
  • MIRN509-3
• Synonyms
  • hsa-mir-509-3
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126337
• RefSeq identity
  • NR_030629
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:31640
• Gene
  MIR7-3
• Long gene name
  microRNA 7-3
• Synonyms gene
  • MIRN7-3
• Synonyms
  • hsa-mir-7-3
• Locus
  19p13.3
• Discovery year
  2004-04-23
• Entrez gene record
  407045
• RefSeq identity
  • NR_029607
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:37518
• Gene
  PIRC14
• Long gene name
  piwi-interacting RNA cluster 14
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313784
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37516
• Gene
  PIRC12
• Long gene name
  piwi-interacting RNA cluster 12
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313808
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:31646
• Gene
  MIR9-3
• Long gene name
  microRNA 9-3
• Synonyms gene
  • MIRN9-3
• Synonyms
  • hsa-mir-9-3
• Locus
  15q26.1
• Discovery year
  2004-04-23
• Entrez gene record
  407051
• RefSeq identity
  • NR_029692
• Classification
  • MicroRNA MIR9 family

Gene info

• Identity
  HGNC:33686
• Gene
  MIR941-3
• Long gene name
  microRNA 941-3
• Synonyms gene
  • MIRN941-3
• Synonyms
  • hsa-mir-941-3
• Locus
  20q13.33
• Discovery year
  2007-10-24
• Entrez gene record
  100126352
• RefSeq identity
  • NR_030639
• Classification
  • MicroRNAs

Gene info

• Identity
  HGNC:37519
• Gene
  PIRC15
• Long gene name
  piwi-interacting RNA cluster 15
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313848
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

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