TKL1, transketolase (chloroplastic)

• Catalog number
  AS15 2903
• Price
  Please ask
• Size
  50 µl

• Available ordering format
  Lyophilized
• Immunogen
  Purified, recombinant mature TKL1 of Arabidopsis thaliana, UniProt: Q8RWV0, TAIR: At3g60750
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 50 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), immunoprecipitation (IP)
• Connected products
  AS15 2873 | anti-SBP | Sedoheptulose-1,7-bis phosphatase, rabbit antibodiescollection of antibodies to carbohydrate metabolismPlant protein extraction buffer Secondary antibodies
• Recommended dilutions for use
  1:2000 with ECL (WB)
• Molecular weight expected аpparent
  72 kDa
• Verified reactivity
  Arabidopsis thaliana, Pisum sativum
• Possible reactivity
  Synechococcus elongatus sp PCC 7942
• No reactivity
  to be determined
• Supplementary information
  TKL1 has MW of 79.28 kDa with and 73.45 kDa without presequence; about 72 kDa on SDS gelAntibody works on whole leaf extracts and isolated chloroplasts and is also recognizing recombinant TKL protein.
• References
  Rocha et al. (2014). Phosphorylation of Arabidopsis transketolase at Ser428 provides a potential paradigm for the metabolic control of chloroplast carbon metabolism. Biochem J. 2014 Mar 1;458(2):313-22. doi: 10.1042/BJ20130631.
• Scientific context
  TKL-1 (Transketolase-1, chloroplastic) is involved in the pathway of Calvin cycle. This enzyme is catalyzing the reversible transfer of a two-carbon ketol group from fructose-6-phopshate or sedoheptuloze-7-phosphate to glyceraldehyde-3-phosphate to yield xylulose-5-phosphate and erythrose-4-phosphate or ribose-5-phosphate. AtTKL1 is expressed in highest levels in photosynthetic tissue, while AtTKL2 is expressed mainly during embryo development. Alternative name: TK.
• Notes
  Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
• Protein number
  Q8RWV0
• TAIR number
  At3g59780
• Hyperlink

• Gene target
  TKL1   transketolase   chloroplastic  
• Short name
  TKL1, transketolase (chloroplastic)
• Alternative name
  TKL1, transketolase (chloroplastic)

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