-
Available ordering format
Lyophilized
-
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SVR4, UniProt Q9M0H2, TAIR: At4g28590
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Serum
-
How to reconstitute
For reconstitution add 50 µl of sterile water.
-
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applications
western blot (WB)
-
Connected products
AS13 2726 | SVR4-like | Supressor of variegation 4 - like, rabbit antibodyPlant protein extraction buffer Secondary antibodies
-
Recommended dilutions for use
1 : 1000 - 1: 5000 with standard ECL (WB)
-
Molecular weight expected аpparent
34.8 | 19 kDa
-
Verified reactivity
Arabidopsis thaliana, Hordeum vulgare
-
Possible reactivity
please, inquire
-
No reactivity
no confirmed exceptions from predicted reactivity are currently known
-
Supplementary information
-
References
Powikrowska et al. (2013). SVR4 (suppressor of variegation 4) and SVR4-like: two proteins with a rolein proper organization of the chloroplast genetic machinery. Physiol Plant. Sep 23. doi: 10.1111/ppl.12108.
-
Scientific context
SVR4 (Supressor of variegation 4) is a nuclear-encoded chloroplast-located protein required for proper function of the plastid transcriptional machinery. Synonymes: ATECB1, EARLY CHLOROPLAST BIOGENESIS 1, ECB1, MESOPHYLL-CELL RNAI LIBRARY LINE 7, MRL7.
-
Notes
This protein is present in very low amounts only in early stages of plant development and this has to be taken into account when harvesting the tissue. Western blots were done on: Arabidopsis thaliana total protein extract from cotyledons and Hordeum vulgare (2-14 days old plants).
-
Protein number
Q9M0H2
-
TAIR number
At4g28590
-
