SPS, Sucrose phosphate synthase, global
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Catalog numberAS03 035A
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PricePlease ask
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Size200 µg
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Available ordering formatLyophilized in PBS pH 7.4
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ImmunogenKLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences, including Arabidopsis thaliana isoforms 1F Q94BT0, 2F, 3F and 4F. Oryza sativa Q67WN8, Solanum tuberosum Q43845
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified serum
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How to reconstituteFor reconstitution add 100 µl of sterile water
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Storage conditionstore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applicationswestern blot (WB)
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Connected productsAS06 185 | anti-SPS sucrose phosphate antibody, maizePlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use1: 1000 - 1: 5000 with standard ECL (WB)
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Molecular weight expected аpparent120 | 120-130 kDa (fragments of 30/90 kDa may be detected)
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Verified reactivityArabidopsis thaliana, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Solanum tuberosum, Triticum aestivum, Pinus strobus, Zea mays
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Possible reactivityBrassica napus, Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Populus balsamifera, Robinia pseudoacaci, Ricinus communis, Saccharum officinarum, Solanum lycopersicum, Theobroma cacao, Vicia faba, Vitis vinifera
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationPeptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants.
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ReferencesZhang et al. (2014). Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014 Aug 27. pii: S0014-5793(14)00621-8. doi: 10.1016/j.febslet.2014.08.019.
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Scientific contextSPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.
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NotesNone
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Protein numberQ94BT0 , Q67WN8 , Q43845
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TAIR numberAT5G20280
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Gene target
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Gene symbolSEPHS1, SMS
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Short nameSPS, Sucrose phosphate synthase, global
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Alternative nameSPS, Sucrose phosphate synthase, global
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Gene info
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Identity
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Gene
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Long gene nameselenophosphate synthetase 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namespermine synthase
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Synonyms gene
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Synonyms gene name
- Snyder-Robinson X-linked mental retardation syndrome
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-11-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Seven-beta-strand methyltransferase motif containing
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VEGA ID
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Locus Specific Databases