SPS, Sucrose phosphate synthase, global

  • Catalog number
    AS03 035A
  • Price
    Please ask
  • Size
    200 µg
  • Available ordering format
    Lyophilized in PBS pH 7.4
  • Immunogen
    KLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences, including Arabidopsis thaliana isoforms 1F Q94BT0, 2F, 3F and 4F. Oryza sativa Q67WN8, Solanum tuberosum Q43845
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified serum
  • How to reconstitute
    For reconstitution add 100 µl of sterile water
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    AS06 185 | anti-SPS sucrose phosphate antibody, maizePlant protein extraction bufferSecondary antibodies
  • Recommended dilutions for use
    1: 1000 - 1: 5000 with standard ECL (WB)
  • Molecular weight expected аpparent
    120 | 120-130 kDa (fragments of 30/90 kDa may be detected)
  • Verified reactivity
    Arabidopsis thaliana, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Solanum tuberosum, Triticum aestivum, Pinus strobus, Zea mays
  • Possible reactivity
    Brassica napus, Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Populus balsamifera, Robinia pseudoacaci, Ricinus communis, Saccharum officinarum, Solanum lycopersicum, Theobroma cacao, Vicia faba, Vitis vinifera
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants.
  • References
    Zhang et al. (2014). Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014 Aug 27. pii: S0014-5793(14)00621-8. doi: 10.1016/j.febslet.2014.08.019.
  • Scientific context
    SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.
  • Notes
    None
  • Protein number
    Q94BT0 , Q67WN8 , Q43845
  • TAIR number
    AT5G20280
  • Gene target
  • Gene symbol
    SEPHS1, SMS
  • Short name
    SPS, Sucrose phosphate synthase, global
  • Alternative name
    SPS, Sucrose phosphate synthase, global
Gene info
Gene info
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