SIP1, Small basic intrinsic protein 1-1

• Catalog number
  AS09 495
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Inquire
• Immunogen
  KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana SIP1;1 Q9M8W5, At3g04090
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
• Verified applications
  ELISA (ELISA), western blot (WB)
• Connected products
  AS09 496 | anti-AtSIP1;1collection of antibodies to endomembrane system Plant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
• Molecular weight expected аpparent
  25.7 | 25 kDa
• Verified reactivity
  Arabidopsis thaliana
• Possible reactivity
  to be determined
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
• References
  Ishikawa et al. (2005). Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana. FEBS Lett. 579:5814-5820.
• Scientific context
  SIP protein functions as a water channel to facilitate the transport of water across cell membrane. It is mainly localized to ER. Alternative name: small basic intrinistic protein 1-1
• Notes
  0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Anti-SIP1;1 antibodies will not recognize SIP2;1 protein.Method for plant ER isolation is available here.
• Protein number
  Q9M8W5
• TAIR number
  AT2G36830, AT3G04090
• Hyperlink
• Description
  Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.

• Gene target
  SIP1   intrinsic   protein   1-1  
• Gene symbol
  ZEB2, IGHVII-1-1, MIR1-1, GEMIN2, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
• Short name
  SIP1, intrinsic protein 1-1
• Alternative name
  SIP1, Small basic intrinsic protein 1-1

Gene info

• Identity
  HGNC:14881
• Gene
  ZEB2
• Long gene name
  zinc finger E-box binding homeobox 2
• Synonyms gene
  • ZFHX1B
• Synonyms gene name
  • zinc finger homeobox 1b
• Synonyms
  • KIAA0569
  • SIP-1
  • SIP1
• Synonyms name
  • SMAD interacting protein 1
• GenBank acession
  • AB011141
• Locus
  2q22.3
• Discovery year
  2001-03-14
• Entrez gene record
  9839
• RefSeq identity
  • NM_014795
• Classification
  • ZF class homeoboxes and pseudogenes
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000187624

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:10884
• Gene
  GEMIN2
• Long gene name
  gem nuclear organelle associated protein 2
• Synonyms gene
  • SIP1
• Synonyms gene name
  • survival of motor neuron protein interacting protein 1
  • gem (nuclear organelle) associated protein 2
• GenBank acession
  • AF027150
• Locus
  14q21.1
• Discovery year
  1998-10-16
• Entrez gene record
  8487
• Pubmed identfication
  • 9323130
  • 9323129
  • 11121410
• Classification
  • Gemins
• VEGA ID
  OTTHUMG00000028816

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:35281
• Gene
  MIR1289-1
• Long gene name
  microRNA 1289-1
• Synonyms gene
  • MIRN1289-1
• Synonyms
  • hsa-mir-1289-1
• Locus
  20q11.22
• Discovery year
  2008-11-03
• Entrez gene record
  100302125
• RefSeq identity
  • NR_031620
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:31488
• Gene
  MIR101-1
• Long gene name
  microRNA 101-1
• Synonyms gene
  • MIRN101-1
• Synonyms
  • hsa-mir-101-1
• Locus
  1p31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406893
• RefSeq identity
  • NR_029516
• Classification
  • MicroRNA MIR101 family

Gene info

• Identity
  HGNC:31545
• Gene
  MIR16-1
• Long gene name
  microRNA 16-1
• Synonyms gene
  • MIRN16-1
• Synonyms
  • hsa-mir-16-1
• Locus
  13q14.2
• Discovery year
  2004-04-23
• Entrez gene record
  406950
• RefSeq identity
  • NR_029486
• Classification
  • MicroRNA MIR15/16 family

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