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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
Purified SHMT protein from Spinacia oleracea
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgG
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS05 074 | anti-GDC-H rabbit antibody, marker of mitochondrial matrixAS06 203A | anti- Idh | isocytrate dehydrogenase rabbit antibody, marker of mitochondrial matrixAS07 212 | anti-VDAC1 marker antibody for mitochondrial outer membranePlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1:5 000 with standard ECL (WB)
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Molecular weight expected аpparent
53 kDa (Arabidopsis thaliana)
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Verified reactivity
Arabidopsis thaliana, Pisum sativum, Spinacia oleracea, Vicia faba
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Possible reactivity
dicots including: Glycine max, Medicago truncatula, Ricinus communis, Solanum tuberosum, Vitis vinifera, monocots: Hordeum vulgare, trees: Populus balsamifera, algae: Chlamydomonas reinhardii
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
this antibody can be used on total leaf extracts and isolated mitochondria
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References
Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba-…L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544. Wei et al. (2013). Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana. Arch Biochem Biophys 1;536(1):87-96.Camejo et al. (2012). Salinity-induced changes in S-nitrosylation of pea mitochondrial proteins. J. Proteomics Dec.11.
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Scientific context
Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.
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Notes
None
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Protein number
AT4G37930
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TAIR number
AT4G37930
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Description
Serine protease, D- or L-serine arginine rich enzyme of serine threonine kinase with serine that is encoded by the codons UCU, UCC, UCA, UCG, AGU and AGC is an ɑ-amino acid that is used in the biosynthesis of proteins. It contains an α-amino group (which is in the protonated −NH+
3 form under biological conditions), a carboxyl group. It is non-essential in humans, meaning the body can synthesize it.
