Sheep, L), TRITC conjugated

  • Catalog number
    AS10 1539
  • Price
    Please ask
  • Size
    1 mg
  • Available ordering format
    Lyophilized
  • Immunogen
    Purified rabbit IgG (H&L)
  • Raised in
    Sheep
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified sheep IgG
  • How to reconstitute
    For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
  • Storage condition
    Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Verified applications
    Contact Gentaur support center
  • Connected products
    other fluorescence conjugated secondary antibodies against rabbit IgG (H&L)
  • Recommended dilutions for use
    This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2000 for most applications.
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    to be determined
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Contact Gentaur support center at [email protected]
  • References
    Refer to PubMed
  • Scientific context
    Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
  • Notes
    Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Gene target
  • Short name
    , L), TRITC conjugated
  • Technique
    antibody Conjugates
  • Host
    Sheep
  • Label
    tritc
  • Species
    Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
  • Alternative name
    ovine, L), TRITC coupled
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