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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from all known sequences of RbcS from monocots and dicots including RuBisCO small subunit 1B of Arabidopsis thaliana At5g38430
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS07 259A | anti-RbcS | Rubisco small subunit (SSU) (affinity purified) rabbit antibodies AS03 037 | anti-RbcL | Rubisco large subunit, form I and form II (50 µl), rabbit antibodiesAS03 037A | anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified), rabbit antibodiesAS03 037-HRP| anti-RbcL | Rubisco large subunit, form I and form II (40 µg, HRP-conjugated), rabbit antibodiesAS15 2955 | anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibodiesAS15 2955S | RbcL II | Rubisco form II positive control/quantitation standardAS01 017 | anti-RbcL | Rubisco large subunit, form I, chicken antibodiesAS01 017S | Rubisco protein standard for quantitative western blot or positive controlAS03 037PRE | Rubisco large subunit, pre-immune serumAS09 409 | Rubisco quantitation kit AS15 2994 | Rubisco ELISA quantitation kit AS07 218 | anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein AS07 222 | anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibodiesmatching secondary antibody
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Recommended dilutions for use
1: 5000 with standard ECL (WB)
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Molecular weight expected аpparent
20 | 15 kDa
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Verified reactivity
Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Malus domestica, Nicotiana tabacum
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Possible reactivity
Camellia oleifera, Erythranthe guttata, Flaveria bidentis, Flaveria sonorensis, Glycine max, L, Nicotiana benthamiana, Oryza sativa, Petunia hybrida, Polianthes tuberosa, Populus deltoides, Triticum aestivum, Solanum melongena, Solanum tuberosum, Zea mays, liverwort Marchantia paleacea, green algae
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No reactivity
cyanobacteria
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Supplementary information
This product can be sold containing ProClin if requested.
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References
Robert et al. (2015). Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein. Plant Biotechnol J. 2015 Aug 19. doi: 10.1111/pbi.12452. Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479. Krasuska et al. (2015). Switch from heterotrophy to autotrophy of apple cotyledons depends on NO signal. Planta. 2015 Jul 18. Huang et al. (2015). Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis. Volume 236, July 2015, Pages 185-194. Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.Sun et al. (2014). The response of rbcL, rbcS and rca genes in cucumber, (Cucumis sativus L.) to growth and induction light intensity. Acta Physiol Plant, October 2014, Volume 36, Issue 10, pp 2779-2791
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Scientific context
Rubisco catalyzes the rate-limiting step of CO2 fixation in photosynthesis. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes.
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Notes
None
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Protein number
P10796
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TAIR number
AT5G38430
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