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Available ordering format
Lyophilized
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Immunogen
Purified goat IgG, whole molecule.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store freeze-dried powder at 2-8 °C.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
other anti-goat DyLight conjugated secondary antibodies
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Recommended dilutions for use
1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Goat IgG (H&L)
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Rabbit anti-Goat IgG (H&L), DyLight® 488 conjugated, min. cross-reactivity to human, mouse, rat lgG is a secondary antibody conjugated to DyLight® 488, which binds to Goat IgG (H&L) in immunological assays.DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries
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Notes
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Goat IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on goat IgG - light chains on all goat immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin goat serum immunoglobulins - IgG from human, mouse or rat .
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
