Rabbit, L), Biotin conjugated
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Catalog number
AS16 3306
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Price
Please ask
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Size
2.0 mg
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Available ordering format
Liquid
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Immunogen
Purified Hamster IgG, whole molecule
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8 °C.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
other secondary antibodies to hamster IgG
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Recommended dilutions for use
1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Hamster IgG (H&L)
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Rabbit anti-Hamster IgG (H&L) is a secondary antibody, conjugated to biotin, which binds to hamster IgG (H&L) in immunological assays.
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Notes
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Hamster IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on hamster IgG - light chains on all hamster immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin hamster serum immunoglobulins .
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Short name
Rabbit, L), conjugated
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Technique
Rabbit, antibody Conjugates
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Host
Rabbit, Rabbits
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Label
Biotin
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Alternative name
production species: rabbit, L), biotinilated coupled
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Alternative technique
rabbit-anti
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