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Available ordering format
Lyophilized
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Immunogen
Purified Bovine IgG, whole molecule
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store freeze-dried powder at 2-8 °C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
other anti-bovine, dylight conjugated, secondary antibodies
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Recommended dilutions for use
1:20 - 1:2 000 (FACS, IM), optimal working dilution should be determined by the investigator.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Bovine IgG
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Rabbit anti-Bovine IgG (H&L), DyLight® 633 conjugated is a secondary antibody conjugated to DyLight® 633, which binds to Bovine IgG (H&L) in immunological assays. DyLight® 633 has Amax = 638 nm, Emax = 658 nm.DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
Antibody purity is > 95% based on SDS-PAGE,Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on IEP, this antibody reacts with: · heavy (gamma) chains on bovine IgG · light chains on all bovine immunoglobulins.Based on IEP, no reactivity is observed to: · non-immunoglobulin bovine serum immunoglobulins.
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Protein number
DAA31645
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TAIR number
Refer to NCBI
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
