Rabbit, DyLight® 550 conjugated,min. cross-reactivity to human serum

  • Catalog number
    AS12 1957
  • Price
    Please ask
  • Size
    1 mg
  • Available ordering format
    Lyophilized
  • Immunogen
    Purified Mouse IgG, whole molecule
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified Rabbit IgG
  • How to reconstitute
    For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
  • Storage condition
    Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Verified applications
    Contact Gentaur support center
  • Connected products
    other dylight anti-mouse secondary antibodies
  • Recommended dilutions for use
    1:20 - 1:2,000 for most applications
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    to be determined
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins human serum proteins.
  • References
    to be added when available
  • Scientific context
    Rabbit anti-mouse IgG (H&L) - DyLight®550 Conjugate, min. cross-reactivity to human serum is a secondary antibody conjugated to DyLight® 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
  • Notes
    Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
  • Latin name
    Oryctolagus cuniculus
  • Description
    Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
  • Group
    Sera
  • Gene target
    DyLight®   550   conjugated   min   cross  
  • Short name
    Rabbit, DyLight® 550 conjugated min. cross-reactivity serum
  • Technique
    Rabbit, serum, antibody Conjugates
  • Host
    Rabbit, Rabbits
  • Label
    DyLight
  • Species
    Humans
  • Alternative name
    production species: rabbit, DyLight® 550 coupled,min. cross-This (antibody to-) recognizes to H. sapiens blood serum
  • Alternative technique
    rabbit-anti, sera
  • Tissue
    serum
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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