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Available ordering format
Lyophilized
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Immunogen
Purified goat IgG, whole molecule.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store freeze-dried powder at 2-8 °C.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
other anti-goat DyLight conjugated secondary antibodies
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Recommended dilutions for use
1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Goat IgG (H&L)
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Rabbit anti-Goat IgG (H&L), DyLight® 550 conjugated is a secondary antibody conjugated to DyLight® 550, which binds to Goat IgG (H&L) in immunological assays.DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Goat IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on goat IgG - light chains on all goat immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin goat serum immunoglobulins .
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
