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Available ordering format
Lyophilized
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Immunogen
Part of Arabidopsis thaliana recombinant plastid terminal oxidase, TAIR: At4g22260, UniProt: Q56X52
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 100 µl of sterile water.
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB)
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Connected products
AS10 1625 | Anti-FNR | Ferredoxin-NADP+-oxidoreductase (higher plants), rabbit antibodies other antibodies to proteins involved in photosynthetic electron transferSecondary antibodies
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Recommended dilutions for use
1: 4000 with ECL (WB)
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Molecular weight expected аpparent
30 | 37-41 kDa (Arabidopsis thaliana)
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Verified reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii
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Possible reactivity
Higher plants
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No reactivity
There is not known exception at this moment
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Supplementary information
In most plants it is a minor polypeptide and consequently enrichment by analyzing membrane fractions for example is recommended.
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References
Cournac et al. (2000b). Flexibility in photosynthetic electron transport: a newly identified chloroplast oxidase involved in chlororespiration. Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1447-54 Josse et al. (2000). A Plastid Terminal Oxidase Associated with Carotenoid Desaturation during Chromoplast Differentiation. Plant Physiol. 2000 Aug;123(4):1427-36 Cournac et al. (2000a). Electron flow between photosystem II and oxygen in chloroplasts of photosystem I-deficient algae is mediated by a quinol oxidase involved in chlororespiration. J Biol Chem. 2000 Jun 9;275(23):17256-62.
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Scientific context
PTOX (plastid terminal oxidase) is a component of electron transfer chain responsible for desaturation of phytoene, which prevents the generation of reactive oxygen species. It is involved in the differentiation of plastids: chloroplasts, amyloplasts, and etioplasts. PTOX is expressed ubiquitously in plant tissues and is located in the lumen.Synonymes: IM, IM1, immutants, AOX4, alternative oxidase 4, ubiquinol oxidase 4, chloroplastic/chromoplastic
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Notes
None
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Protein number
Q56X52
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TAIR number
At4g22260
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