PsaC, PSI-C core subunit of photosystem I (PsaC antibody + PsaC protein positive control )
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Catalog numberAS04 042P
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PricePlease ask
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Size50 µl antibody, 100 µl protein standard
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Available ordering formatLyophilized
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ImmunogenKLH-conjugated synthetic peptide conserved in all known PsaC proteins includingArabidopsis thaliana AtCg01060, Hordeum vulgare P69416, Oryza sativa P0C360, Chlamydomonas reinhardtii Q00914, Synechococcus elongatus Q31QV2
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationSerum
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionstore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applicationswestern blot (WB)
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Connected productsAS04 042S | PsaC protein standard for quantitationcollection of antibodies to PSI proteinsPlant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use1: 1000 with ECL (WB)
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Molecular weight expected аpparent9 kDa
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Verified reactivityArabidopsis thaliana, Horderum vulgare, Spinacia oleracea, Synechococcus PCC 7942, Cyanophora paradoxa, Heterosigma akashiwo, Thalassiosira pseudonan, Euglena gracilis, Micromonas pusilla, Chlamydomonas reinhardtii, Porphyra sp., Gonyaulax polyedra, Emiliania huxleyi
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Possible reactivitydicots including Glycine max, Nicotiana tabacum, Spinacia oleracea, and monocots, Physcomitrella patens, algae and cyanobacteria, Prochlorococcus sp. (surface and a deep water ecotype)
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationIn some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.Protein standard: use a load of 2 µl per well with ECL detection system and 4 µl per well with alkaline phospatase.
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ReferencesIfuku et al. (2005). PsbP protein, but not PsbQ protein, is essential for the regulation and stabilization of photosystem II in higher plants. Plant Physiol. 3:1175-1184. Oesterhelt et al (2007). Regulation of photosynthesis in the unicellular acidophilic red alga Galdieria sulphuraria. Plant J.3:500511.
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Scientific contextPsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10 kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe-S clusters FA and FB through two cysteine-rich domains.
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NotesPeptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine and isoleucine variants. For reconstitution of PsaC antibodies (AS10 939) add 50 µl of sterile water. For reconstitution of PsaC positive control add 100 µl of sterile water.
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Protein numberP62090 , P69416 , P0C360 , Q00914 , Q31QV2
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TAIR numberATCG01060
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PropertiesIf you buy Antibodies supplied by agisera they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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DescriptionIsotype or positive controls by peptides, antibodies and deactivated samples. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
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Grouppositif
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French translationanticorps
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Gene target
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Gene symbolNTF6A, SMC4, PCK1, MYB, SFTPC, VCX3B, MAF, RFX1, EGR4
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Short namePsaC, PSI-C core subunit of photosystem I (PsaC antibody + PsaC protein positive control )
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TechniqueAntibody, Positive, Control, antibodies against human proteins, antibodies for, controls
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Alternative namePsaC, PSI-C core functionnal sequence on photosystem I (PsaC (antibody to-) + PsaC protein positive reference )
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Alternative techniquecontrols, antibodies
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Tissuecontrol
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Gene info
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Identity
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Gene
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Long gene nameneurotrophin 6 alpha (pseudogene)
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-10-15
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Entrez gene record
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Pubmed identfication
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Classification
- Neurotrophins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namestructural maintenance of chromosomes 4
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Synonyms gene
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Synonyms gene name
- SMC4 (structural maintenance of chromosomes 4, yeast)-like 1
- SMC4 structural maintenance of chromosomes 4-like 1 (yeast)
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-22
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Entrez gene record
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Pubmed identfication
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Classification
- Structural maintenance of chromosomes proteins
- Condensin I subunits
- Condensin II subunits
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namephosphoenolpyruvate carboxykinase 1
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Synonyms gene name
- phosphoenolpyruvate carboxykinase 1 (soluble)
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Synonyms
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Locus
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Discovery year1990-09-10
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMYB proto-oncogene, transcription factor
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Synonyms gene name
- v-myb avian myeloblastosis viral oncogene homolog
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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Classification
- Myb/SANT domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesurfactant protein C
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Synonyms gene
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Synonyms gene name
- surfactant, pulmonary-associated protein C
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Synonyms
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Synonyms name
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Locus
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Discovery year1988-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- BRICHOS domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namevariable charge X-linked 3B
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Synonyms
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Locus
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Discovery year2005-01-11
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Classification
- Variable charge X/Y family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMAF bZIP transcription factor
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Synonyms gene name
- v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog
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Synonyms
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Locus
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Discovery year1991-08-01
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Entrez gene record
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Pubmed identfication
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Classification
- Basic leucine zipper proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameregulatory factor X1
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Synonyms gene name
- regulatory factor X, 1 (influences HLA class II expression)
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Regulatory factor X family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameearly growth response 4
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Synonyms
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Locus
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Discovery year1992-07-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
