PR-2 | GLU I, Class I beta-1,3-glucanase

• Catalog number
  AS07 208
• Price
  Please ask
• Size
  2 mg

• Available ordering format
  Lyophilized
• Immunogen
  Purified tobacco class I, basic ß-1,3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLB P27666 derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991). Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988).
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Total IgG in PBS pH 7.4 (without Ca++)
• How to reconstitute
  For reconstitution add 100 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), immunolocalization (IL)
• Connected products
  AS10 687 | PR-1 | Pathogenesis-related protein 1, rabbit antibodies AS12 2366 | PR-2 | pathogenesis-related protein 2, rabbit antibodies for Arabidopsis thalianaAS07 207 | PR-3 | CHN | class I chitinase, rabbit antibodiesAS12 2369 | PR-4 | Pathogenesis-related protein 4, rabbit antibodiesAS12 2373 | PR-5 | Pathogenesis-related protein 5, rabbit antibodiescollection of antibodies to other proteins involved in a response to pathogen attackSecondary antibodies
• Recommended dilutions for use
  8 ug/ml with standard ECL (WB)
• Molecular weight expected аpparent
  37 | 33 kDa
• Verified reactivity
  Nicotiana benthamian, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, Phalenopsis Sogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
• Possible reactivity
  dicots
• No reactivity
  Arabidopsis thaliana
• Supplementary information
  Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.
• References
  Wang et al. (2014). Elicitation of Hypersensitive Responses in Nicotiana glutinosa by the Suppressor of RNA Silencing Protein P0 from Poleroviruses. Mol Plant Pathol. 2014 Sep 4. doi: 10.1111/mpp.12201. Huey-wen et al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014. Munger et al. (2012). Beneficial -˜unintended effects-™ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biol. 2012 Nov 1;12:198. doi: 10.1186/1471-2229-12-198.
• Scientific context
  Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase
• Notes
  for more details on immunolocalization, please referr to Keefe et al (1990). Plant 182: 43-51This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.
• Protein number
  P27666
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  PR-2   GLU   Class   beta-1   3-glucanase  
• Gene symbol
  TRE-TTC2-2, PRH2, TRE-TTC8-2, TRE-CTC1-2, TMEM37, PGR, TRE-TTC4-2, TRE-TTC1-2, TRE-TTC2-1
• Short name
  PR-2 | GLU I, Class I beta-1 3-glucanase
• Alternative name
  PR-2 | L-gutamic acid I, Class I b-1,3-glucanase

Gene info

• Identity
  HGNC:34622
• Gene
  TRE-TTC2-2
• Long gene name
  tRNA-Glu (anticodon TTC) 2-2
• Synonyms gene
  • TRNAE11
• Synonyms gene name
  • transfer RNA glutamic acid 11 (anticodon UUC)
  • transfer RNA-Glu (TTC) 2-2
• Synonyms
  • tRNA-Glu-TTC-2-2
• GenBank acession
  • HG983780
• Locus
  15q12
• Discovery year
  2008-08-29
• Entrez gene record
  100189063
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:9367
• Gene
  PRH2
• Long gene name
  proline rich protein HaeIII subfamily 2
• Synonyms
  • Pr
• Synonyms name
  • parotid proline-rich protein
  • acidic salivary proline-rich protein, HaeIII type, 2
• Locus
  12p13.2
• Discovery year
  2001-06-22
• Entrez gene record
  5555
• Pubmed identfication
  • 3009472
• RefSeq identity
  • NM_001110213
• Classification
  • Proline rich proteins
• VEGA ID
  OTTHUMG00000168556

Gene info

• Identity
  HGNC:54204
• Gene
  TRE-TTC8-2
• Long gene name
  tRNA-Glu (TTC) 8-2
• Synonyms
  • tRNA-Glu-TTC-8-2
• Locus
  1p22.1
• Discovery year
  2019-03-28
• Classification
  • Low confidence cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34651
• Gene
  TRE-CTC1-2
• Long gene name
  tRNA-Glu (anticodon CTC) 1-2
• Synonyms gene
  • TRNAE13
• Synonyms gene name
  • transfer RNA glutamic acid 13 (anticodon CUC)
  • transfer RNA-Glu (CTC) 1-2
• Synonyms
  • tRNA-Glu-CTC-1-2
• GenBank acession
  • HG983754
• Locus
  1q23.3
• Discovery year
  2008-08-29
• Entrez gene record
  100189091
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:18216
• Gene
  TMEM37
• Long gene name
  transmembrane protein 37
• Synonyms
  • PR1
  • PR
• GenBank acession
  • AF361356
• Locus
  2q14.2
• Discovery year
  2004-12-14
• Entrez gene record
  140738
• RefSeq identity
  • NM_183240
• VEGA ID
  OTTHUMG00000153304

Gene info

• Identity
  HGNC:8910
• Gene
  PGR
• Long gene name
  progesterone receptor
• Synonyms
  • PR
  • NR3C3
• GenBank acession
  • M15716
• Locus
  11q22.1
• Discovery year
  2001-06-22
• Entrez gene record
  5241
• RefSeq identity
  • NM_000926
• Classification
  • Nuclear receptor subfamily 3 group C
• VEGA ID
  OTTHUMG00000167531

Gene info

• Identity
  HGNC:34976
• Gene
  TRE-TTC4-2
• Long gene name
  tRNA-Glu (anticodon TTC) 4-2
• Synonyms gene
  • TRNAE23
• Synonyms gene name
  • transfer RNA glutamic acid 23 (anticodon UUC)
  • transfer RNA-Glu (TTC) 4-2
• Synonyms
  • tRNA-Glu-TTC-4-2
• GenBank acession
  • HG983783
• Locus
  1q23.3
• Discovery year
  2008-08-29
• Entrez gene record
  100189407
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34901
• Gene
  TRE-TTC1-2
• Long gene name
  tRNA-Glu (anticodon TTC) 1-2
• Synonyms gene
  • TRNAE20
  • TRE-TTC1-1
• Synonyms gene name
  • transfer RNA glutamic acid 20 (anticodon UUC)
  • transfer RNA-Glu (TTC) 1-1
  • transfer RNA-Glu (TTC) 1-2
• Synonyms
  • tRNA-Glu-TTC-1-2
• GenBank acession
  • HG983777
• Locus
  13q14.11
• Discovery year
  2008-08-29
• Entrez gene record
  100189333
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34589
• Gene
  TRE-TTC2-1
• Long gene name
  tRNA-Glu (anticodon TTC) 2-1
• Synonyms gene
  • TRNAE7
• Synonyms gene name
  • transfer RNA glutamic acid 7 (anticodon UUC)
  • transfer RNA-Glu (TTC) 2-1
• Synonyms
  • tRNA-Glu-TTC-2-1
• GenBank acession
  • HG983779
• Locus
  13q14.12
• Discovery year
  2008-08-29
• Entrez gene record
  100189030
• Classification
  • Cytoplasmic transfer RNAs

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