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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide derived from Raphanus sativus PAQ2 O80369
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
ELISA (ELISA), western blot (WB)
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Connected products
collection of antibodies to plasma membrane proteins AS09 489 | anti-PIP1;1, PIP1;2, PIP1;3 | aquaporinsAS09 490 | anti-PIP2;2 | plasma membrane aquaporin 2bAS09 491 | anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3Plant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent
30.34 | 28 kDa (Raphanus sativus)
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Verified reactivity
Arabidopsis thaliana, Oryza sativa, Raphanus sativus
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Possible reactivity
dicots including: Brassica napus, Gossypium mexicanum,
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Peptide used to elicit this antibody is also conserved in Arabidopsis thaliana PIP2;1,PIP2;2, PIP2;3 and this antibody reacted also with PIP2;2 in experiments with knock out plants.Manufactured by Operon Biotechnology
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References
Markus G. et al., (2004). Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters.Mol. Biol. Cell. 15, 3393-3405.
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Scientific context
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane.
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Notes
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for plasma membrane preparation can be found here.
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Protein number
O80369
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TAIR number
AT3G53420
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