PEPCK, PEP carboxy kinase

• Catalog number
  AS07 241
• Price
  Please ask
• Size
  200 µl

• Available ordering format
  Lyophilized
• Immunogen
  KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 200 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  AS07 242 | anti-GOGAT | glutamine oxoglutarate aminotransferasePlant and algal protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1 : 1000 with standard ECL (WB)
• Molecular weight expected аpparent
  73 | 78 kDa
• Verified reactivity
  Ananas comosus, Miscantus giganteus, Panicum virgatum, Phaseolus vulgaris, Saccharum spp. hybrid clone C91-301, Spartina alterniflora , Spartina patens, Zea mays, mice
• Possible reactivity
  Brassica napus, Chromera velia, Cucumis sativus, Flaveria sp., Lycopersicon esculentum, Medicago sativa, Oryza sativa, Panicum maximum, Urochloa panicoides, Zoysia japonica , Zea mays
• No reactivity
  Arabidopsis thaliana
• Supplementary information
  Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.Antibody can be also used following 2D gel electrophoresis.
• References
  AragoÌn et al. (2013). The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles. Plant Cell Rep. Aug 20. (Ananas comosus, western blot detection following 2D gel electrophoresis)
• Scientific context
  Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) Is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2.
• Notes
  None
• Protein number
  Q9SLZ0
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  PEPCK   PEP   carboxy   kinase  
• Gene symbol
  PCK1, PCP4, PCK2, PAEP
• Short name
  PEPCK, PEP carboxy kinase
• Alternative name
  PEPCK, PEP carboxy phosphorylation catalyst

Gene info

• Identity
  HGNC:8724
• Gene
  PCK1
• Long gene name
  phosphoenolpyruvate carboxykinase 1
• Synonyms gene name
  • phosphoenolpyruvate carboxykinase 1 (soluble)
• Synonyms
  • PEPCK-C
• Locus
  20q13.31
• Discovery year
  1990-09-10
• Entrez gene record
  5105
• Pubmed identfication
  • 1492743
• VEGA ID
  OTTHUMG00000032825

Gene info

• Identity
  HGNC:8742
• Gene
  PCP4
• Long gene name
  Purkinje cell protein 4
• Synonyms
  • PEP-19
• GenBank acession
  • X93349
  • U53709
• Locus
  21q22.2
• Discovery year
  1997-06-09
• Entrez gene record
  5121
• Pubmed identfication
  • 8931698
  • 8914602
• RefSeq identity
  • NM_006198
• VEGA ID
  OTTHUMG00000086731

Gene info

• Identity
  HGNC:8725
• Gene
  PCK2
• Long gene name
  phosphoenolpyruvate carboxykinase 2, mitochondrial
• Synonyms gene name
  • phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Synonyms
  • PEPCK
  • PEPCK2
• GenBank acession
  • AK129934
• Locus
  14q11.2-q12
• Discovery year
  1993-03-04
• Entrez gene record
  5106
• Pubmed identfication
  • 8645161
  • 9657976
• RefSeq identity
  • NM_001018073
• VEGA ID
  OTTHUMG00000028791

Gene info

• Identity
  HGNC:8573
• Gene
  PAEP
• Long gene name
  progestagen associated endometrial protein
• Synonyms gene name
  • progestagen-associated endometrial protein
• Synonyms
  • PEP
  • PP14
  • GdA
  • GdS
  • GdF
  • PAEG
  • GD
  • MGC138509
  • MGC142288
• Synonyms name
  • glycodelin-A
  • glycodelin-S
  • glycodelin-F
  • progesterone-associated endometrial protein
  • glycodelin
  • PP14 protein (placental protein 14)
  • pregnancy-associated endometrial alpha-2-globulin
  • alpha uterine protein
• Locus
  9q34.3
• Discovery year
  1991-07-25
• Entrez gene record
  5047
• Pubmed identfication
  • 3320533
  • 2016092
• RefSeq identity
  • NM_001018049
• Classification
  • Lipocalins
• VEGA ID
  OTTHUMG00000020914

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