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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PDR8, Q9XIE2, At1g59870
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
ELISA (ELISA), western blot (WB)
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Connected products
collection of antibodies to plant plasma membrane proteinsPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent
165 |150 kDa (Arabidopsis thaliana)
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Arabidopsis thaliana
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No reactivity
Triticum sp.
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Supplementary information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Protocol for preparation of plant plasma membranes can be found under "more information" tab. Additoinal 160 kDa cross-reacting band is detected when working with chloroplast fractions.Manufactured by Operon Biotechnologies.
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References
Campe at al. (2015). ABC transporter PEN3/PDR8/ABCG36 interacts with calmodulin that, like PEN3, is required for Arabidopsis nonhost resistance. New Phytol. 2015 Aug 28. doi: 10.1111/nph.13582.Yoshihiro et al. (2006). Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection. Plant Cell Physiol. 47:309-318.
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Scientific context
PDR8 protein is a key factor which controls the extent of cell death in defence response. Alternative names: ABC transporter ABCG.36, AtABCG36, pleiotropic drug resistance protein 8, protein PENETRATION 3
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Notes
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Plant plasma membrane isolation protocol can be found here.
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Protein number
Q9XIE2
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TAIR number
AT1G59870
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